纳米氧化铝对大鼠肾脏的潜在毒性作用-东南大学学报医学版

纳米氧化铝对大鼠肾脏的潜在毒性作用

单位：江苏省疾病预防控制中心

分类号：

出版年·卷·期（页码）：2011·30·第一期（39-42）

摘要：

[目的] 探讨纳米氧化铝颗粒对大鼠肾脏的潜在毒性作用。[方法] 将75只SD大鼠随机分为对照组、非纳米氧化铝组（nNAOs）（50mg/kg）、纳米氧化铝组(NAOs)（50mg/kg），每组25只，每2 d腹腔注射1次，共60 d。分别在第2、4、8、15、30d每组取3只大鼠，采用原子吸收分光光度法测定铝元素在肾组织中的含量。注射60d后，全自动生化仪测定大鼠血清生化指标，免疫组织化学染色观察肾组织中巢蛋白（nestin）表达水平。[结果] 纳米氧化铝组大鼠肾组织中铝元素的浓度在染毒2d后即显著升高，第15d达到峰值后逐渐降低。而非纳米氧化铝组铝元素的含量随染毒时间延长而逐渐升高，在30d达到高峰；与对照组相比，纳米氧化铝组可引起血清UA（尿酸）升高（P＜0.05），而非纳米氧化铝组可引起CRE(肌酐)升高（P＜0.05）；三组大鼠肾小球中均可见nestin蛋白表达，阳性细胞胞体形态及数目未见显著差异。[结论] 纳米氧化铝与非纳米氧化铝在大鼠肾脏中排泄特征不同，分别引起血清不同指标的异常升高，但在免疫组化切片中未见肾小球组织器质性损害。

[Objective] To determine the toxic effects of nano-scale aluminum oxide (NAOs) on kidney in rats. [Method]: SD male rats were randomly divided into 3 groups: control group, non nano-scale aluminum oxide (nNAOs) group and NAOs group. Each group was treated by intraperitoneal injection once two days. The accumulation and metabolism of Al was analyzed by atomic absorption spectrum (AAS), expression of nestin was analyzed by immunohistochemistry. After 60 days the rats were sacrificed and the serum were collected for biochemical analysis. [Results]: The accumulation and metabolism of NAOs and nNAOs in rat kidneys were different. Compared with control group, the concentration of serum UA in NAOs group and the serum CRE in nNAOs group were significant higher. There is no different expression of nestin in the three groups. [Conclusion]: NAOs may induce the damage to the kidneys in rats, which maybe related to the special particle and metabolism characters.

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