Rats are widely used for research in partial hepatectomy. Open liver resection by conventional open laparotomy has become the classic technique; Microsurgery can individualized dissect and ligate the vascular and biliary branches of the rat liver, it improved the technique of partial hepatectomy of rat; laparoscopic partial hepatectomy in the rat is feasible, safe and reproducible，it is a valuable tool for further basic research into laparoscopic surgery as well as for other investigations of liver regeneration