辛伐他汀对HT29细胞的促凋亡作用及可能机制-东南大学学报医学版

辛伐他汀对HT29细胞的促凋亡作用及可能机制

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学附属中大医院消化科, 江苏南京 210009

分类号：R965; R979.1

出版年·卷·期（页码）：2012·31·第三期（323-326）

摘要：

目的:研究辛伐他汀对HT29细胞的促凋亡作用,以及可能存在的调控机制。方法:将人结肠癌HT29细胞在不同浓度辛伐他汀的培养基中培养24、48、72 h,观察细胞的增殖情况,MTT法检测其抑制率;流式细胞术检测不同浓度辛伐他汀对HT29细胞凋亡率的影响,RT-PCR法观察HT29细胞Bcl-2、Bax mRNA水平,蛋白质印迹法观察HT29细胞Bcl-2、Bax蛋白表达情况。结果:不同浓度辛伐他汀对HT29细胞增殖有抑制作用,且其抑制作用同时存在浓度及时间效应(P<0.01);辛伐他汀对HT29细胞有促凋亡作用,抑制率随药物浓度增大而增加(P<0.05);PCR、蛋白质印迹法检测表明辛伐他汀可分别在基因及蛋白水平下调Bcl-2的表达及上调Bax的表达。结论:辛伐他汀可显著抑制HT29细胞的增殖,促进其凋亡,其可能与下调Bcl-2基因、上调Bax基因的表达有关。

Objective: Evaluate the effect of simvastatin on the apoptosis in HT29 cells and its possible mechanisms of anticancer effects. Methods: In vitro, human colon cancer HT29 cells were treated by simvastatin in a series of concertrations. The absorbance value was detected by means of MTT assay and growth inhibition rate was calculate. Cell apoptosis was assessed by flow cytometry. The expression of Bcl-2 and bax were analyzed by RT-PCR and Western blotting. Results: Simvastatin could inhibit the growth of the human colon cancer HT29 cells and the inhibitory effect was dose-and-time dependent(P<0.01). Comparing with control group, the HT29 cell apoptosis was induced by simvastatin at 48 hours, the cell apoptosis rate was gradually increased by simvastatin(P<0.05).The expression of Bcl-2 was downregulated and bax was upregulated by simvastain at 48 hours. Conclusions: Simvastatin could inhibit the growth of hunman colon cancer HT29 cells in a dose-and-time dependent manner, its possible mechanisms could be inducing apoptosis through downregulating Bcl-2 expression and upregulating bax expression.

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