人脐带间充质干细胞对新生鼠脑白质软化灶的神经保护作用-东南大学学报医学版

人脐带间充质干细胞对新生鼠脑白质软化灶的神经保护作用

单位：1. 东南大学医学院江苏南京 210009;
2. 江苏省干细胞工程技术研究中心,江苏泰州 225300

分类号：R-332; R741.05

出版年·卷·期（页码）：2012·31·第四期（430-435）

摘要：

目的:探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUcMSCs),对新生未成熟鼠脑室周围白质软化灶受损神经细胞的保护作用。方法:建立新生大鼠PVL(periventricular leukomalacia)动物模型,造模后随机分为两组。于第5天(P5d)分别给两组大鼠腹腔注射5-溴脱氧尿核苷(5-bromodeoxyuridine,BrdU)标记的第二代HUcMSCs[每只1×10⁷(0.05 ml)^-1]或0.05 ml PBS无菌溶液。24 h后(P6d)处死一组大鼠,行BrdU染色观察HUcMSCs在鼠脑内的迁移;另一组于移植HUcMSCs后14 d(P19d)行微型MRI(MicroMRI)扫描观察受损区域修复程度,髓磷脂碱性蛋白(myelin basic protein,MBP)染色观察移植HUcMSCs对大鼠脑室周围脑白质损伤的保护。结果:HUcMSCs在宿主体内明显向侧脑室周围白质损伤区域迁移,且脑室周围存活少突胶质细胞数量明显多于注射PBS组,微型MRI扫描观察到HUcMSCs可缩小受损脑白质病灶,远期神经行为学观察到HUcMSCs移植组神经行为学高于PBS组(P<0.05)。结论:HUcMSCs对新生大鼠PVL具有神经保护作用,可缩小病变范围。

Objective: To investigate the behavior of the homing of the human umbilical cord mesenchymal stem cells (HUcMSCs)after intraperitoneal implantation to the premature rats with periventricular leukomalacia(PVL), observe the injury volume of preterm mouse with PVL by MicroMRI and explore the neuroprotective effect. Methods: HUcMSCs were cultured in vitro, the second generation was selected. In one group,every mouse with PVL was injected HUcMSCs stained-BrdU (bromodeoxyuridine)(1×10⁷/0.05 ml) intraperitoneally after operation. The other group was inject the 0.05 ml bioclean PBS intraperitoneally simultaneously.The mice of two groups were put to death after 24 hours, the movement of the HUcMSCs to the damaged host brain region was observed with BrdU immunofluorescence staining, and the reduction of the injury volume after injection of HUcMSCs was measured by MBP(myelin basic protein) staining and MicroMRI scanning. Long-term neuropathology of the two groups was tracked after two weeks. Results: The HUcMSCs migrating to the host brain damage region decreased the injury volume. The amount of the survival gliocytes increased and the long-term neuropathology was improved more efficiently than that of control animals (P<0.05). Conclusion: HUcMSCs have neuroprotective effect on neotal mouce with PVL, and reduce infarct volume.

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