4-苯乙烯磺酸马来酸酐共聚物抑制HSV-2感染研究-东南大学学报医学版

4-苯乙烯磺酸马来酸酐共聚物抑制HSV-2感染研究

单位：南京大学医学院公共健康医学中心,江苏南京 210093

关键词：4-苯乙烯磺酸马来酸酐共聚物单纯疱疹病毒2型 HSV-2阴道感染小鼠模型抗病毒

分类号：R-33

出版年·卷·期（页码）：2012·31·第五期（563-566）

摘要：

目的:探索4-苯乙烯磺酸马来酸酐共聚物(PSM)对单纯疱疹病毒Ⅱ型(HSV-2)感染细胞或小鼠的抑制作用。方法:建立Vero-ICP10细胞系,通过Luciferase检测不同浓度PSM对感染细胞中HSV-2的抑制作用,并通过In cell western技术检测HSV-2 gD蛋白表达,确定不同PSM浓度对HSV-2 gD蛋白表达的抑制作用;建立HSV-2阴道感染小鼠模型,观察PSM对HSV-2经生殖道感染小鼠的抑制作用。结果:Luciferase实验和In cell western实验结果均表明PSM具有抗HSV-2感染细胞的作用;小鼠模型中,5%的PSM能有效防止小鼠感染HSV-2(P<0.001)。结论:PSM在体内和体外模型中均可以抑制HSV-2感染。

Objective: To investigate the antiviral effect of polymer of styrenesulfonic acid and maleic acid (4-styrenesulfonic acid-co-maleic acid) (PSM) against herpes simplex virus type 2 (HSV-2). Methods: A Vero-ICP10 cell line containing virus-controlled Luciferase as indicator was established for analyzing the effect of different concentrations of PSM on HSV-2 infection. An In cell western assay was also used for detecting intracellular viral protein expression. PSM inhibitory activity was further evaluated in a HSV-2 vaginal challenge murine model. Results: The results of in vitro analyses showed that PSM could protect cell from HSV-2 infection, and the murine model showed that 5% PSM protected 95% of the animals from HSV-2 infection (P<0.001). Conclusion: PSM is a potential agent for the prevention of HSV-2 infection through sexual route.

参考文献：

[1] ANDERSON R A, FEATHERGILL K, DIAO X, et al. Evaluation of poly(styrene-4-sulfonate) as a preventive agent for conception and sexually transmitted diseases[J]. Androl, 2000,21:862-875.
[2] BABA M, NAKAJIMA M, SCHOLS D, et al. Pentosan polysulfate, a sulfated oligosaccharide, is a potent and selective anti-HIV agent in vitro[J]. Antiviral Res, 1988,9(6):335-343.
[3] ZHU J, AURELIAN L. AP-1 cis-response elements are involved in basal expression and Vmw 110 transactivation of the large subunit of herpes simplex virus type 2 ribonucleotide reductase(ICP10)[J].Virology, 1997,231(2):301-312.
[4] KOELLE D M, COREY L. Recent progress in herpes simplex virus immunobiology and vaccine research[J]. Clin Microbiol Rev, 2003,16:96-113.
[5] GILLGRASS A E, ASHKAR A A, ROSENTHAL K L, et al. Prolonged exposure to progesterone prevents induction of protective mucosal responses following intravaginal immunization with attenuated herpes simplex virus type 2[J]. J Virol,2003,77:9845-9851.
[6] LEKSTROM-HIMES J A, WANG K, PESNICAK L, et al. The comparative biology of latent herpes simplex virus type 1 and type 2 infections:latency-associated transcript promoter activity and expression in vitro and in infected mice[J].J Neurovirol,1998,4(1):27-37.
[7] 丁洁,周静,袁榴娣.果蝇S2细胞中高表达荧光素酶重组质粒的构建及活性测定[J].东南大学学报:医学版,2006,25(6):403-406.
[8] EGORINA E M, SOVERSHAEV M A, OSTERUD B. In cell western assay: a new approach to visualize tissue factor in human monocytes[J].J Thromb Haemost, 2006,4(3):614-620.
[9] MCLEAN C S, ERTURK M, JENNINGS R, et al.Protective vaccination against primary and recurrent disease caused by herpes simplex virus (HSV) type-2 using a genetically disabled HSV-1[J]. Infect Dis, 1994,170:1100-1109.
[10] 刘庆芝,姚吉龙,都红蕾,等.全反式维甲酸对裸鼠子宫内膜癌治疗作用的实验研究[J].东南大学学报:医学版,2011,30(2):278-282.
[11] ZHU J, HLADIK F, WOODWARD A, et al. Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisition[J].Nature Medicine, 2009,15:886-892.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录