环孢菌素A、汉防己甲素及两者联合逆转K562/A02 细胞的多药耐药-东南大学学报医学版

环孢菌素A、汉防己甲素及两者联合逆转K562/A02 细胞的多药耐药

作者：郭晶晶¹ 陈宝安¹ 高峰¹ 丁家华¹ 许文林² 沈惠玲² 孙新臣³ 成红艳³ 高冲¹ 孙耘玉¹ 程坚¹ 王骏¹ 赵刚¹ 宋慧

单位：1.东南大学附属中大医院,血液科,江苏,南京,210009； 2.江苏大学附属人民医院,血液科,江苏,镇江,212002； 3.东南大学附属中大医院,肿瘤科,江苏,南京,210009

分类号：R733.7, R969, R979.1

出版年·卷·期（页码）：2008·27·第二期（82-86）

摘要：

目的:探讨环孢菌素A(CsA)、汉防己甲素(Tet)及两者联合应用对人白血病耐药细胞系K562/A02多药耐药的逆转作用.方法:采用MTT法测定柔红霉素(DNR)对细胞的半数抑制量(IC50),流式细胞仪检测细胞凋亡率和细胞内DNR浓度.结果:DNR对K562细胞和K562/A02细胞IC50分别为0.27和21.22 mg·L-1;CsA(1 mg·L-1)及Tet(0.1 mg·L-1)单独和联合处理K562/A02后,DNR的IC50分别为7.83、5.32和2.32 mg·L-1;CsA和Tet对DNR作用K562的IC50无明显影响;K562/A02细胞48 h凋亡率为2.19%,DNR(1 mg·L-1)作用K562/A02细胞凋亡率为2.70%,CsA和Tet单独及联合处理后凋亡率分别为10.27%、17.64%和52.79%;两药处理后细胞内化疗药物DNR浓度亦明显增加.结论:CsA及Tet均可逆转耐药,且联合作用逆转效果更强.

Objective To study the reversible effect of cyclosporine A(CsA),tetrandrine(Tet) and their combination on multidrug resistance of cell line K562/A02.Methods The IC50 of DNR was determined by MTT assay.The apoptotic changes and the intracellular DNR concentration were observed by flow cytometry.Results Treating K562/A02 cells with CsA(1 mg·L-1),Tet(0.1 mg·L-1)and both for 48 hours partially restored the sensitivity of K562/A02 cells to DNR,but had no effect on K562 cells;K562/A02 cells showed apoptotic characteristics after treated with CsA and both for 48 hours;CsA and Tet(alone or combination) elevated the intracellular DNR concentration in K562/A02 cells.Conclusion Multidrug resistance can be partially reversed by CsA or Tet,the combination of both drugs shows a great reversal effect.

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