超声微泡辅助干细胞移植治疗急性心肌梗死的实验研究-东南大学学报医学版

超声微泡辅助干细胞移植治疗急性心肌梗死的实验研究

作者：徐琢¹ 冯毅¹ 童嘉毅¹ 李金鹆¹ 马根山¹ 沈成兴¹ 祁春梅¹ 苏亚民¹ 张晓黎¹ 杨芳² 张宇²

单位：1.东南大学,临床医学院,江苏,南京,210009； 2.东南大学,生物科学与医学工程学院,江苏,南京,210096

分类号：R-33, R541.4, R454.3, Q813

出版年·卷·期（页码）：2008·27·第三期（192-194）

摘要：

目的:探讨超声微泡在自体骨髓间充质干细胞(BMMSCs)移植治疗猪急性心肌梗死(AMI)改善心功能中的作用.方法:选取14只3～4月龄小型家猪,抽取骨髓,分离、培养、标记BMMSCs,球囊封堵猪左冠状动脉前降支(LAD)建立AMI模型,分为实验组(超声微泡作用下移植干细胞,n=8)和对照组(无超声微泡作用,n=6),7d后向LAD梗死处输注自体BMMSCs移植治疗.在移植前1d和移植后6周分别行64层螺旋CT心脏扫描及组织病理学检查.结果:与对照组相比,实验组心功能改善更为明显,梗死区毛细血管数(16.37±3.20 vs 9.50±1.87,P<0.05)、普鲁士蓝染色阳性细胞数(71.63±14.09 vs 40.67±9.48,P<0.05)、结蛋白免疫组化染色阳性细胞数(31.88±4.16 vs 19.00±2.28,P<0.05)均明显增加.结论:超声微泡增效自体BMMSCs移植治疗AMI,能更有效地改善心肌梗死后的心功能,促进血管新生.

Objective To study effects of ultrasound-mediated microbubble destruction in myocardium-targeted delivery of autologus bone marrow mesenchymal stem cells(BMMSCs) to treat acute myocardial infarction by improving heart function.Methods BMMSCs of 14 Chinese minipigs,aged 3-4 months old,were selected,cultured and labeled by suerparamagnetic iron oxide.AMI model was made by blocking blood stream of left anterior descending branch.Pigs were devided into 2 groups:experiment group(BMMSCs+Utrasound+Bubble,n=8) and control group(BMMSCs,n=6).After 7 days,BMMSCs were transplanted into AMI areas of each host heart through intracoronary way.64-slice multidetector computed tomography were performed to observe left ventricular function 1 day before and 6 weeks after transplantion.The histological analysis was conducted.Results Compared to the control group,the heart function was improved more significantinly in the experiment group.The capillary numbers of experiment group were more than those of the control group(16.37±3.20 vs 9.50±1.87,P<0.05),the prussian blue-positive stained cells in experiment group outnumbered those in the control group(71.63±14.09 vs 40.67± 9.48,P<0.05),so did the desmin-positive cells(31.88±4.16 vs 19.00±2.28,P<0.05).ConclusionUltrasound-mediated microbubble destruction shows favorable effects on myocardium delivery of autologus BMMSCs to treat acute myocardial infarction by improving heart function and angiogenesis.

参考文献：

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