P-gp、MRP、Bcl-2、Bax在膀胱癌中的表达及其意义-东南大学学报医学版

P-gp、MRP、Bcl-2、Bax在膀胱癌中的表达及其意义

单位：东南大学附属中大医院,泌尿外科,江苏,南京,210009

分类号：R737.14, Q344.13

出版年·卷·期（页码）：2007·26·第四期（279-282）

摘要：

目的:探讨P-糖蛋白(P-gp)、多药耐药相关蛋白(MRP)、Bcl-2、Bax在膀胱移行细胞癌中的表达及其意义.方法:采用SP免疫组化技术检测59例膀胱移行细胞癌和13例正常膀胱组织标本中P-gp、MRP、Bcl-2、Bax的表达.结果:(1)复发膀胱癌组织中P-gp、MRP、Bcl-2表达水平高于初发癌组织(P＜0.05);Bax表达水平与之相反(P＜0.05).(2)P-gp、Bcl-2表达随着病理分级、分期上升而增高,Bax表达随着病理分级、分期上升而下降,MRP与病理分级、分期不相关.(3)P-gp表达与Bcl-2呈正相关,与Bax呈负相关.结论:膀胱癌组织P-gP、MRP、Bcl-2、Bax可作为判断膀胱癌的恶性度及预后的重要指标.

Objective To investigate the expressions and value of P-glycoprotein(P-gp),multidrug resistance-associated protein(MRP),Bcl-2 and Bax in transitional cell carcinoma of bladder(TCC).Methods The expressions of P-gp,MRP,Bcl-2 and Bax were studied by means of immunohistochemical SP technique in 59 TCCs and 13 controls with normal bladder mucosas.Results(1)the expressions of P-gp,MRP and Bcl-2 in recurrent TCCs were significantly higher than those in primary ones,and the expression of Bax in recurrent TCCs was lower than that in primary ones.(2)In TCCs,the expressions of P-gp and Bcl-2 increased with the increase of grade and stage,while the expressions of Bax decreased with the increase of grade and stage.the expression of MRP has no relationship with the grade and stage.(3)P-gp expression was positively related with the Bcl-2,and negatively with Bax.Conclusion The expression of P-gp,MRP,Bcl-2 and Bax can be used as the important markers of malignancy and prognosis of TCCs.

参考文献：

[1] KLUNDER J W, KOMDEUR R, vail der GRAAF W T. Expression of multidrug resistance-associated proteins in rhabdomyosarcomas before and after chemotherapy:the relationship between lung resistance-related protein(LRP)and differentiation. 2003(2). doi:10.1053/hupa.2003.10
[2] MARZOLINI C, PAUS E, BUCLIN T. Polymorphisms in human MDR1(P-glycoprotein):recent advances and clinical relevance. 2004(1). doi:10.1016/j.clpt.2003.09.012
[3] BENLLOCH M, ORTEGA A, FERRER P. Acceleration of glutathione efflux and inhibition of gamma-glutamyltranspep-tidase sensitize metastatic B16 melanoma cells to endothelium-induced cytotoxicity. 2005(8). doi:10.1074/jbc.M408531200
[4] 郭和清, 王晶瑶, 李贤初. 人膀胱癌非P-糖蛋白介导的多重抗药性. 空军总医院学报2000(1). doi:10.3969/j.issn.2095-3402.2000.01.001
[5] 张磊, 李钢, 史立新. 膀胱癌中Fas、FasL、Bcl-2及Bax的表达及意义. 解放军医学杂志2006(2)
[6] BILIM V, KASAHARA T, NOBORU H. Caspase involved synergistic cytotoxicity of Bcl-2 antisense oligonucleotides and adriamycin on transitional cell cancer cells. 2000(2). doi:10.1016/S0304-3835(0)00428-6
[7] SAKAEDA T, NAKAMURA T, HIRAI M. MDRl up-regulated by apoptotic stimuli suppresses apoptotic signaling. 2002(19). doi:10.1023/A:1020302825511
[8] RAFTOPOULOU M, ETIENNE-MANNEVILLE S, SELF A. Regulation of cell migration by the C2 domain of the tumor suppressor PTEN. 2004(5661). doi:10.1126/science.1092089
[9] WEIS S M, CHERESH D A. Pathophysiological consequences of VEGF-induced vascal permeability. 2005(7058). doi:10.1038/nature03987
[10] SAMPATH J, SUN D, KIDD V J. Mutant p53 cooperates with ETS and selectively up-regulates human MDRl not MRP1. 2001(42). doi:10.1074/jbc.M103429200

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录