MDP-Cyclic与CpG-ODN 1826对小鼠膀胱瘤免疫治疗效应的初步研究-东南大学学报医学版

MDP-Cyclic与CpG-ODN 1826对小鼠膀胱瘤免疫治疗效应的初步研究

单位：东南大学基础医学院,病原生物学与免疫学系,江苏,南京,210009

分类号：R737.14, R730.51, R965

出版年·卷·期（页码）：2006·25·第三期（164-168）

摘要：

目的:研究卡介苗(BCG)有效成分MDP-Cyclic和细菌细胞核有效成分CpG-ODN 1826免疫治疗小鼠膀胱瘤效应.方法:用小鼠膀胱肿瘤细胞(BTT739)在T739小鼠皮下接种,肿瘤形成后,于瘤内多点注射MDP-Cyclic或CpG-ODN 1826共3次.ELISA检测IFN-γ和IL-4含量,3H-TdR掺入法和MTY法检测脾淋巴细胞增殖效应和对靶细胞杀伤效应.结果:(1)与生理盐水(NS)对照组相比,联合应用MDP-Cyclic和CpG-ODN 1826组小鼠肿块重量明显减轻,优于单独使用CpG-ODN 1826组;(2)联合用药能明显促进IFN-γ分泌,而对IL-4没有显著影响;(3)脾淋巴细胞对联合用药或单用MDP-Cyclic刺激均有增殖作用,与NS对照组相比差异有统计学意义;(4)脾淋巴细胞对肿瘤细胞杀伤率联合用药组高于其它组.结论:联合应用免疫调节剂MDP-Cyclic和CpG-ODN 1826能抑制小鼠膀胱肿瘤生长.

Objective To investigate the immunotherapeutic effect of MDP-Cyclic and CpG-ODN 1826,components of BCG and bacteria nucleus respectively,on bladder tumor in mouse model.Methods The model of mouse bladder tumor was established by inoculating mouse bladder tumor cells(BTT739) into T739 mouse.The various drugs were injected into the sites of mouse tumor respectively.Serum cytokines of IFN-γ and IL-4 were detected by ELISA.Spleen lymphocyte proliferation assay and cytotoxicity assay were performed by ~3H-TdR incorporation and MTT respectively.Results The tumor weight of rats treated with MDP-Cyclic and CpG-ODN 1826 was obviously decreased compared with controls,which also was less than that of mice treated only with CpG-ODN 1826.The IFNγ level was in creased significantly in mice treated with two kinds of immunomodifier,but IL-4 secretion was not affected.The spleen lymphocyte proliferation response to MDP-Cyclic or CpG-ODN 1826 was remarkably increased compared with that of control group and the activity of killing tumor cells reached(68.85±15.71)%,higher than those of other groups.Conclusions The immunotherapy of immunomodifier MDP-Cyclic and CpG-ODN 1826 inhibits the growth of bladder tumor in mice.

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