基于甲基乙烯基醚/马来酸酐共聚物水凝胶三维培养卵巢癌细胞的研究-东南大学学报医学版

基于甲基乙烯基醚/马来酸酐共聚物水凝胶三维培养卵巢癌细胞的研究

单位：1. 东南大学医学院病原生物学与免疫学系, 江苏南京 210009;
2. 东南大学生物科学与医学工程学院, 江苏南京 210096;
3. 东南大学附属中大医院肿瘤科, 江苏南京 210009

分类号：R737.31;Q813.11

出版年·卷·期（页码）：2014·33·第一期（21-26）

摘要：

目的：验证卵巢癌SKOV-3细胞在自制甲基乙烯基醚/马来酸酐共聚物水凝胶中培养形成的3D模型。方法：通过SKOV-3细胞生长曲线、迁移和侵袭能力、耐药实验等，对比自制的水凝胶培养基与市售BME胶和胶原蛋白胶Ⅰ胶培养基对SKOV-3细胞若干特性的影响。结果：SKOV-3细胞在自制水凝胶TZ028和TZ029培养基第10~15天时，为3D细胞培养最佳时期；在Transwell实验中表现出与胶原蛋白Ⅰ胶相似的细胞侵袭力，并优于BME培养基；在紫杉醇耐药实验中形成细胞3D结构耐药性要高于2D细胞培养（P<0.05）；与BME和胶原蛋白Ⅰ胶培养基相比，该细胞对相同浓度下紫杉醇耐药性差异无统计学意义（P >0.05）。结论：自制的甲基乙烯基醚/马来酸共聚物和交联剂聚乙二醇交联反应形成的3D培养基质水凝胶，与商品化BME胶和胶原蛋白胶Ⅰ胶培养基相比，具有类似的细胞培养功能。

Objective: To validate ovarian cancer cells cultured in three-dimensional(3D) culture based on poly(ethylene glycol)-cross linked poly(methyl vinyl ether-co-maleic acid) hydrogels developed by our group. Methods: The abilities of proliferation, migration, invasion and resistant to paclitaxel of SKOV-3 cells were respectively evaluated in our developed poly(methyl vinyl ether-co-maleic acid)(PMVE-co-MA)-based hydrogel compared to basal medium eagle(BME) and collagenⅠ gel 3D culture. Results: The best growth period of SKOV-3 cells in the developed hydrogel of TZ028 or TZ029 3D culture was 15-20 days. The result of Transwell assay shows that the invasion ability of SKOV-3 cells in the developed hydrogel 3D culture appeared similar result as in collagenⅠ gel and better than BME 3D culture.The resistance to paclitaxel of SKOV-3 cells indicated that the formed 3D structure cells in the developed hydrogel of TZ028 or TZ029 exhibited high resistance compared with in 2D culture(P<0.05).There was no obvious different resistance to paclitaxel of SKOV-3 cells between the TZ028 or TZ029 3D culture and the BME or the collagenⅠ gel 3D culture(P >0.05). Conclusion: The developed poly(ethylene glycol)-cross linked poly(methyl vinyl ether-co-maleic acid) hydrogel 3D culture has the similar cellular culture function as the commercialized BME and collagenⅠ gel 3D culture.

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