CD14启动子基因多态性与中国高原藏族人群胃癌易感性研究-东南大学学报医学版

CD14启动子基因多态性与中国高原藏族人群胃癌易感性研究

单位：1. 兰州军区兰州总医院血液科, 甘肃兰州 730050;
2. 兰州军区兰州总医院乳腺外科, 甘肃兰州 730050;
3. 西藏消化病研究所, 西藏拉萨 850000

分类号：R394

出版年·卷·期（页码）：2015·34·第二期（177-181）

摘要：

目的:研究分化抗原簇14(cluster of differentiation 14,CD14)启动子基因多态性与青藏高原藏族人群胃癌易感性的相关性.方法:采用聚合酶链反应-连接酶反应(PCR-LDR)技术对186例藏族胃癌患者和216例藏族正常对照人群CD14启动子基因多态性进行分析,并就特定基因型与胃癌发病风险及生存进行分析.结果:在胃癌组和对照组中,CD14 rs5744455C>T位点的等位基因T的频率分别是40.8%和20.0%,基因型TT的频率分别是20.0%和1.8%,等位基T和基因型TT增加了胃癌的发病风险;而rs59568848 C >T位点的基因型CT频率分别是34.5%和74.5%,CT明显降低了胃癌的发病风险,胃癌组基因型TT和等位基因C、T的频率与对照组之间无明显差异.结论:CD14启动子区rs5744455 C/T等位基因T和基因型TT明显增加藏族人群胃癌的风险,而rs59568848C >T中基因型CT则明显减少胃癌的发生.

Objective: To investigate the association between gastric cancer and the polymorphism of cluster of differentiation 14(CD14) to find susceptibility genes and protective genes in Chinese Tibetan population from Tibet plateau. Methods: The CD14 between Tibetan patients with gastric cancer(n=186) and Tibetan control population(n=216) were detected by polymerase chain reaction with ligase detection reaction(PCR-LDR). Association of genotypes with the risk of gastric cancer was analyzed. Results: The genotypic frequency of allele T on CD14 rs5744455C >T in patient groups and control groups were 40.8% and 20.0%, respectively, meanwhile the frequency of genetype TT in both groups were 20.0% and 1.8%, indicating that both allele T and genetype TT significantly reduced the risk of gastric cancer in Tibetan. Statistical analysis also revealed significantly decreased frequencies of genetype CT on CD14 rs59568848 C/T(34.5% vs 74.5%, P <0.001, OR=0.10, 95%CI:0.03-0.31) in patients with gastric cancer when compared with control group. Conclusion: Both allele T and genetype TT of CD14 rs5744455C >T increase the risk of gastric cancer whereas genetype CT of CD14 rs59568848 C/T might contribute to protection in Chinese Tibetan population.

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