C-反应蛋白全身性敲除大鼠模型的繁育及鉴定-东南大学学报医学版

C-反应蛋白全身性敲除大鼠模型的繁育及鉴定

单位：1. 南京医科大学代谢疾病研究中心, 江苏南京 210029;
2. 华南理工大学生物科学与工程学院前孵化器中心, 广东广州 510006

分类号：R-33

出版年·卷·期（页码）：2016·35·第三期（346-349）

摘要：

目的:繁育及鉴定C-反应蛋白(CRP)全身性敲除的大鼠模型。方法:通过TALEN技术构建CRP敲除的杂合子大鼠,并将F1代雌鼠与雄鼠相互交配得到F2代大鼠。通过DNA测序以及ELISA法鉴定CRP敲除大鼠。结果:通过测序发现CRP敲除大鼠的CRP基因1#外显子缺失了TTGGT共5个碱基,ELISA结果显示CRP敲除大鼠中血清CRP含量较野生型相比下调98%。结论:成功构建了CRP全身性敲除大鼠,为后期CRP生物学功能的相关研究提供了动物模型。

Objective:To breed and identify rat C-reactive protein (CRP) knock out models. Methods:The rat CRP knock out heterozygotes models were constructed with TALEN technology, and F2 generation was got by F1 generation copulation. The identification of rat CRP knock out was done by DNA sequencing and ELISA assays. Results:CRP knock out rats had five bases missing in their exon1#. The CRP level of the knock out rats displayed 98% reduction compared with the wild types. Conclusion:We have successfully constructed CRP knock out rat models, which is critical animals for the studies of biological functions of CRP.

参考文献：

[1] TILLETT W S,FRANCIS T.Serological reactions in pneumonia with a non-protein somatic fraction of pneumococcus[J].J Exp Med,1930,52(4):561-571.
[2] 纪国业,王斌,杜江,等.不同时间血液灌流对脓毒症兔血IL-6及TNF-α水平的影响[J].现代医学,2011,39(2):129-133.
[3] THOMPSON D,PEPYS M B,WOOD S P.The physiological structure of human C-reactive protein and its complex with phosphocholine[J].Structure,1999,7(2):169-177.
[4] PEPYS M B,HIRSCHFIELD G M.C-reactive protein:a critical update[J].J Clin Invest,2003,111(12):1805-1812.
[5] RIDKER P M,CUSHMAN M,STAMPFER M J,et al.Inflammation,aspirin,and the risk of cardiovascular disease in apparently healthy men[J].N Engl J Med,1997,336(14):973-979.
[6] DEHGHAN A,KARDYS I,de MAAT M P,et al.Genetic variation,C-reactive protein levels,and incidence of diabetes[J].Diabetes,2007,56(3):872-878.
[7] PRADHAN A D,MANSON J E,RIFAI N,et al.C-reactive protein,interleukin 6,and risk of developing type 2 diabetes mellitus[J].JAMA,2001,286(3):327-334.
[8] 洪永青,朱蓉,孟自力.血清炎性因子CRP、IL-6和TNF-α在慢性阻塞性肺疾病继发肺动脉高压中的研究[J].中国现代医学杂志,2013(33):27-31.
[9] CHANG M K,BINDER C J,TORZEWSKI M,et al.C-reactive protein binds to both oxidized LDL and apoptotic cells through recognition of a common ligand:phosphorylcholine of oxidized phospholipids[J].Proc Natl Acad Sci USA,2002,99(20):13043-13048.
[10] STRANG F,SCHUNKERT H.C-reactive protein and coronary heart disease:all said——is not it?[J].Mediators Inflamm,2014,2014:1-7.
[11] VOLANAKIS J E,KAPLAN M H.Specificity of C-reactive protein for choline phosphate residues of pneumococcal C-polysaccharide[J].Proc Soc Exp Biol Med,1971,136(2):612-614.
[12] de BEER F C,BALTZ M L,MUNN E A,et al.Isolation and characterization of C-reactive protein and serum amyloid P component in the rat[J].Immunology,1982,45(1):55-70.
[13] TEUPSER D,WEBER O,RAO T N,et al.No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice[J].J Biol Chem,2011,286(8):6272-6279.
[14] TRION A,de MAAT M P,JUKEMA J W,et al.No effect of C-reactive protein on early atherosclerosis development in apolipoprotein E*3-leiden/human C-reactive protein transgenic mice[J].Arterioscler Thromb Vasc Biol,2005,25(8):1635-1640.
[15] HIRSCHFIELD G M,GALLIMORE J R,KAHAN M C,et al.Transgenic human C-reactive protein is not proatherogenic in apolipoprotein E-deficient mice[J].Proc Natl Acad Sci USA,2005,102(23):8309-8314.
[16] SIMONS J P,LOEFFLER J M,AL-SHAWI R,et al.C-reactive protein is essential for innate resistance to pneumococcal infection[J].Immunology,2014,142(3):414-420.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录