长链非编码RNA CCAT1对前列腺癌PC-3细胞增殖、迁移及凋亡的影响-东南大学学报医学版

长链非编码RNA CCAT1对前列腺癌PC-3细胞增殖、迁移及凋亡的影响

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学附属中大医院泌尿外科, 江苏南京 210009

分类号：R737.25

出版年·卷·期（页码）：2017·36·第一期（1-4）

摘要：

目的：探讨长链非编码RNA结肠癌相关转录因子1（CCAT1）对前列腺癌PC-3细胞增殖、迁移及凋亡的影响。方法：采用荧光定量PCR检测CCAT1 siRNA在PC-3细胞中的敲低效率；通过CCK-8法、transwell迁移实验、流式细胞仪（FACS）检测低表达CCAT1对PC-3细胞增殖、迁移及凋亡的影响。结果：PC-3细胞转染CCAT1 siRNA后，qPCR检测细胞中CCAT1表达量明显降低；低表达CCAT1能显著抑制细胞增殖，减弱迁移能力，促进细胞凋亡。结论：CCAT1可能通过对细胞增殖、迁移及凋亡的调控促进前列腺癌发生、发展。

Objective:To explore the effect of IncRNA colon cancer associated transcript 1(CCAT1) on the proliferation,migration and apoptosis of prostate cancer PC-3 cells.Methods:qPCR assay was performed to confirm the low expression level of CCAT1 after the transfection of CCAT1 siRNA.CCK-8 assay,transwell migration assay and flow cytometry (FACS) were used to observe on its role in cell proliferation,migration and apoptosis.Results:The expression level of CCAT1 was efficiently knocked down with the transfection of CCAT1 siRNA.And knockdown CCAT1 could significantly suppress the ability of proliferation,migration and promote apoptosis.Conclusion:CCAT1 may promote the carcinogenesis of prostate cancer through regulation of cell proliferation,migration and apoptosis.

参考文献：

[1] SIEGEL R L,MILLER KD,JEMAL A.Cancer statistics,2015[J].CA Cancer J Clin,2015,65(1):5-29.
[2] BOSETTI C,BERTUCCIO P,MALVEZZI M,et al.Cancer mortality in Europe, 2005-2009, and an overview of trends since 1980[J].Ann Oncol,2013,24(10):2657-2671.
[3] MALVEZZI M,BERTUCCIO P,ROSSO T,et al.European cancer mortality predictions for the year 2015:does lung cancer have the highest death rate in EU women?[J].Ann Oncol,2015,26(4):779-786.
[4] 陈万青,郑荣寿,张思维,等.2012年中国恶性肿瘤发病和死亡分析[J].中国肿瘤,2016,25(1):1-8.
[5] 韩勖,陈明.长链非编码RNA与肾透明细胞癌的研究进展[J].东南大学学报:医学版,2016,35(3):427-430.
[6] CALEY D P,PINK R C,TRUJILLANO D,et al.Long noncoding RNAs,chromatin,and development[J].Scientific World Journal,2010,10:90-102.
[7] SPIZZO R,ALMEIDA M I,COLOMBATTI A,et al.Long non-coding RNAs and cancer:a new frontier of translational research?[J].Oncogene,2012,31(43):4577-4587.
[8] WANG K C,CHANG H Y.Molecular mechanisms of long noncoding RNAs[J].Mol Cell,2011,43(6):904-914.
[9] WAPINSKI O,CHANG H Y.Long noncoding RNAs and human disease[J].Trends Cell Biol,2011,21(6):354-361.
[10] ZANKE B W,GREENWOOD C M,RANGREJ J,et al.Genome-wide association scan identifies a colorectal cancer susceptibility locus on chromosome 8q24[J].Nat Genet,2007,39(8):989-994.
[11] YANG F,XUE X,BI J,et al.Long noncoding RNA CCAT1,which could be acticated by c-Myc,promotes the progression of gastric carcinoma[J].J Cancer Res Clin Oncol,2013,139(3):437-445.
[12] WILDA M,BUSCH K,KLOSE I,et al.Level of MYC overexpression in pediatric Burkitt's lymphoma is strongly dependent on genomic breakpoint location within the MYC locus[J].Genes Chromosomes Cancer,2004,41(2):178-182.

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