SPIO-shRNA分子探针用于荷瘤裸鼠MR检查的最佳扫描浓度探讨-东南大学学报医学版

SPIO-shRNA分子探针用于荷瘤裸鼠MR检查的最佳扫描浓度探讨

单位：重庆医科大学附属第一医院放射科, 重庆 400016

分类号：R-33;Q6-33

出版年·卷·期（页码）：2017·36·第三期（311-317）

摘要：

目的：探讨SPIO-shRNA分子探针在活体荷瘤裸鼠肿瘤区域组织信号的变化情况，阐明分子探针用于荷瘤裸鼠的最佳扫描浓度。方法：将30只BALB/c荷瘤裸鼠随机分为5组，每组6只，在既定扫描时间（探针注射前及注射后27 h）分别以含铁量为6、12、18、24、30 mg·kg₂^*序列的信号改变最明显；各浓度组比较，肿瘤组织信号强度在18、24、30 mg·kg^-1组较其余组差异明显（P<0.05），而18、24、30 mg·kg^-1组间差异无统计学意义（P>0.05）；HE染色显示肿瘤组织结构紊乱，细胞核异型性较多；普鲁士蓝染色表明不同浓度组肿瘤组织中均存在蓝染的铁颗粒，其中18、24、30 mg·kg^-1组蓝染颗粒分布密集。结论：SPIO-shRNA分子探针能成功靶向于肿瘤组织，且最佳扫描浓度（含铁量）为18 mg·kg^-1。

Objective:To investigate MRI signal variation and the optimal scanning concentration for tumor-bearing nude mice with SPIO-shRNA molecular probes. Methods:Thirty BALB/c tumor-bearing nude mice were randomly divided into five groups with 6 in each group. Iron content of6, 12, 18, 24, 30 mg·kg^-1 was administered respectively through tail vein injection and simultaneously MR examination was performed to measure signal intensity in tumor tissue and contralateral muscle tissue. After treatment, the nude mice were sacrificed and tumor and muscle tissues were sampled for HE and Prussian blue stain. Results:Groups with 6, 12 and 18 mg·kg^-1 were all survived after probe injection, but some nude mice died in 24 and 30 mg·kg^-1 groups after injection or during scanning process. There was no significant difference in tumor tissues volume between the groups. The most marked signal changes were T₂WI and T₂^* GRE and the signal intensity of the tumor tissue in 18, 24 and 30 mg·kg^-1 groups were most obvious(P<0.05), while the 18, 24 and 30 mg·kg^-1 groups showed no statistical difference(P>0.05).HE staining indicated disorganization of tumor tissue as well as marked cell nuclear atypia. Prussian blue staining showed that iron particles were distributed dispersedly in each experimental group, and the most abundant expressions were in 18, 24 and 30 mg·kg^-1 groups. Conclusion:Tumor tissue could be well labeled with SPIO-shRNA molecular probes, and the optimal MR scanning concentration is 18 mg·kg^-1.

参考文献：

[1] 文曦琳,李易,葛晓东,等.卵巢癌特异性MR分子探针的制备[J].基础医学与临床,2014,34(11):1463-1467.
[2] 邓小林,葛晓东,吴晓凤,等.SPIO-shRNA双功能分子探针药动学及活体MR成像研究[J].药学学报,2015(10):1285-1289.
[3] 文曦琳,李易,葛晓东,等.SPIO-PLL-pshRNA分子探针转染卵巢癌SKOV3细胞及MR成像研究[J].重庆医科大学学报,2014(4):506-510.
[4] WANG L,POTTER W M,ZHAO Q.In vivo quantification of SPIO nanoparticles for cell labeling based on MR phase gradient images[J].Contrast Media Mol Imaging,2015,10(1):43-50.图6各浓度组普鲁士蓝染色结果×400Fig 6 Prussian blue straining images(×400)
[5] HAEGELE J,DUSCHKA R L,GRAESER M,et al.Magnetic particle imaging:kinetics of the intravascular signal in vivo[J].Int J Nanomedicine,2014,9:4203-4209.
[6] LI W,ZHANG Z,GORDON A C,et al.SPIO-labeled yttrium microspheres for MR imaging quantification of transcatheter intrahepatic delivery in a rodent model[J].Radiology,2015,278(2):405-412.
[7] 温志鹏,刘海燕,文明,等.SPIO标记的c-erbB2反义探针用于活体荷瘤裸鼠MRI的最佳扫描浓度[J].南方医科大学学报,2013,33(4):496-501.
[8] 温志鹏,刘海燕,文明,等.SPIO标记的c-erbB2反义探针用于活体荷瘤裸鼠MR的最佳扫描时间探讨[J].四川大学学报:医学版,2013,44(5):708-712.
[9] BURNETT J,ROSSI J.RNA-based therapeutics:current progress and future prospects[J].Chem Bio,2012,19(1):60-71.
[10] FARRA R,GRASSI M,GRASSI G,et al.Therapeutic potential of small interfering RNAs/micro interfering RNA in hepatocellular carcinoma[J].World J Gastroenterol,2015,21(30):8994-9001.
[11] MICHELE W,WENLIAN Z,MOHAMMAD H,et al.Magnetic resonance imaging contrast of iron oxide nanoparticles developed for hyperthermia is dominated by iron content[J].Int J Hyperthermia,2014,30(3):192-200.
[12] 李易,文曦琳,孙红武,等.超顺磁性氧化铁(SPIO)与多聚赖氨酸(PLL)纳米粒的制备、结构验证及其空间结构表征[J].复旦学报:医学版,2014,41(3):309-314.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录