白藜芦醇抑制软骨肉瘤细胞SW1353炎症反应的实验研究-东南大学学报医学版

白藜芦醇抑制软骨肉瘤细胞SW1353炎症反应的实验研究

单位：1. 中国医科大学公共卫生学院营养与食品卫生学教研室, 辽宁沈阳 110122;
2. 北华大学公共卫生学院营养与食品卫生学教研室, 吉林吉林 132012;
3. 中国医科大学附属盛京医院骨科, 辽宁沈阳 110004

关键词：软骨内瘤细胞白藜芦醇 Toll样受体4 TIR结构域接头分子髓样分化蛋白88

分类号：R285.5

出版年·卷·期（页码）：2017·36·第三期（337-342）

摘要：

目的：探讨白藜芦醇（RES）对白细胞介素1β（IL-1β）处理的人软骨肉瘤细胞SW1353的效应及其可能机制。方法：采用CCK-8法测定不同浓度IL-1β及RES对SW1353细胞增殖活力的影响。ELISA法检测细胞培养基上清基质金属蛋白酶13（MMP-13）的水平。RT-PCR法检测Toll样受体4（TLR4）、TIR结构域接头分子（TRIF）、髓样分化蛋白88（MyD88） mRNA表达。结果：IL-1β处理对SW1353细胞的增殖活力无明显抑制作用（P>0.05）。RES浓度为6.25、12.5 μmol·L^-1时能促进细胞增殖（P<0.05）。RES浓度在50 μmol·L^-1时对细胞增殖无明显作用（P>0.05），而RES浓度为200 μmol·L^-1时则显著抑制细胞增殖（P<0.05）。IL-1β和RES联合处理与单纯RES处理比较，能够降低细胞增殖活力（P<0.05）。相比对照组，IL-1β处理组培养基上清中MMP-13分泌水平及TLR4、TRIF、MyD88 mRNA表达均明显增加（P<0.01），而RES则能够显著抑制IL-1β诱导的MMP-13的分泌水平，并降低TLR4、TRIF、MyD88 mRNA的表达（P<0.01）。结论：RES可以通过抑制TLR4信号通路对体外培养的SW1353细胞发挥抗骨性关节炎效应。

Objective:To investigate the effect and possible mechanisms of resveratrol on inflammatory response in SW1353 cells induced by IL-1β. Methods:Proliferation ability of SW1353 cell in different concentrations of IL-1β and resveratrol was detected by the CCK-8 assay. The levels of MMP-13 in cell culture supernatants were measured by ELISA. The expressions of TLR4, TRIF and MyD88 mRNA were examined by RT-PCR. Results:CCK-8 assay showed that IL-1β had no obvious effect on SW1353 cells proliferation(P>0.05). At 6.25 and 12.5 μmol·L^-1, resveratrol promoted cell proliferation(P<0.05). There was no discernable inhibition effect on cells with resveratrol at 50 μmol·L^-1(P>0.05). However, resveratrol had significant inhibition effect on cell proliferation when the concentration was 200 μmol·L^-1(P<0.05). In addition,compared with the resveratrol group, cell proliferation ability was reduced in the combination group of IL-1β and resveratrol(P<0.05). The levels of MMP-13 and the expression of TLR4,TRIF and MyD88 mRNA were significantly up-regulated in the presence of IL-1β compared with the control group(P<0.01),while resveratrol treatment could significantly down-regulate them(P<0.01). Conclusion:Resveratrol may exert anti-inflammatory effect on SW1353 cells via the inhibition of TLR4 signaling pathway.

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