大鼠反复轻度脑创伤后外周血中T细胞亚群的变化-东南大学学报医学版

大鼠反复轻度脑创伤后外周血中T细胞亚群的变化

单位：1. 天津医科大学总医院保健医疗部(老年病科), 天津市老年病学研究所, 天津 300052;
2. 天津医科大学总医院神经外科, 天津市神经病学研究所, 天津 300052

分类号：R-33;R651.15

出版年·卷·期（页码）：2017·36·第三期（347-350）

摘要：

目的：探讨大鼠反复轻度脑创伤（rmTBI）后外周血中T细胞亚群的变化及其意义。方法：选择SD大鼠，采用控制性脑皮质撞击法（CCI）制作rmTBI大鼠模型，随机分为假手术对照组以及损伤后1、2、4、6周组共5组，每组8只，通过流式细胞仪检测各组大鼠1、2、4、6周时外周血中CD3⁺、CD4⁺、CD8⁺细胞百分率。结果：CD3⁺ T细胞百分率在1、2周均有显著上升，在4、6周均有显著下降（P<0.05）。CD4⁺ T细胞百分率在伤后持续下降（P<0.05）；CD8⁺ T细胞百分率在伤后1~4周逐渐上升（P<0.05）。结论：rmTBI模型大鼠在伤后存在免疫功能紊乱，这为rmTBI伤后存在外周免疫抑制提供了新的实验证据，其慢性期外周免疫抑制可能对疾病的预后产生重要影响。

Objective:To study kinetics of T lymphocyte subsets in the peripheral blood of rats following repetitive mild traumatic brain injury(rmTBI)and its role in pathogenesis of rmTBI. Methods:Controlled cortical impact(CCI)induced brain injury model. SD rats were randomly assigned to five experimental groups:sham group and 1/2/4/6-week post-injury groups with 8 rats in each group. T lymphocyte subsets in the peripheral blood of the rats were analyzed after rmTBI by flow cytometry. Results:Flow cytometric analysis revealed that a significant increase of T cells(CD3⁺)in the peripheral blood was found at 1/2-week after rmTBI, while the decrease of those was at 4/6-week after rmTBI. The percentage of CD4⁺ T cells decreased continuously during the 6 weeks after rmTBI, while the percentage of CD8⁺ T cells showed upward trend from the 1st week to the 4th week. Conclusion:Immune dysfunction is found in rats after rmTBI compared with normal controls, which provides useful information on peripheral immune suppression in the chronic phase of rmTBI.

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