白头翁皂苷D对人乳腺癌MCF-7细胞中Wnt/β-链蛋白信号通路的影响-东南大学学报医学版

白头翁皂苷D对人乳腺癌MCF-7细胞中Wnt/β-链蛋白信号通路的影响

单位：重庆三峡医药高等专科学校, 重庆市抗肿瘤天然药物工程技术研究中心, 重庆 404120

分类号：R737.9

出版年·卷·期（页码）：2017·36·第三期（379-383）

摘要：

目的：研究白头翁皂苷D对人乳腺癌MCF-7细胞的增殖抑制效应及其机制。方法：不同浓度的白头翁皂苷D作用于人乳腺癌MCF-7细胞48 h后，CCK-8法检测细胞生存抑制率；Hoechst法检测细胞核凋亡变化；蛋白质印迹法检测MCF-7细胞内Wnt/β-链蛋白信号蛋白及下游组件蛋白CyclinD1和c-Myc的表达情况。结果：CCK-8法检测显示白头翁皂苷D可以抑制MCF-7细胞的增殖能力，细胞的生存抑制率呈药物浓度依赖性；Hoechst染色可见部分细胞核的形态发生改变，产生细胞核碎片，核发白等变化；与对照组比较，随着白头翁皂苷D浓度的增加，MCF-7细胞中β-链蛋白的蛋白表达量呈减少趋势；50 nmol·ml^-1的白头翁皂苷D诱导组细胞中CyclinD1及c-Myc的蛋白表达量也明显减少（P<0.05）。结论：白头翁皂苷D可有效抑制人乳腺癌MCF-7细胞的增殖，其机制可能与抑制Wnt/β-链蛋白信号通路的激活，并抑制其下游蛋白CyclinD1和c-Myc的表达有关。

Objective:To investigate the proliferation inhibition effect of pulchinenoside D on human breast carcinoma cell lines MCF-7 in vitro and its mechanism. Methods:The proliferation inhibition rate of cells was detected by CCK-8 assay after the MCF-7 cells were treated with different concentration of pulchinenoside D for 48 h,and the pathological changes of cell nucleus were determined by Hoechst assay. The protein expression of β-catenin,CyclinD1 and c-Myc was examined by Western blot analysis. Results:CCK-8 assay showed that the growth of MCF-7 cells was inhibited by pulchinenoside D in a concentration-dependent manner. Hoechst staining indicated that there were some morphological changes of the cells, such as occurrence of nuclear debris and pale. The expression of β-catenin, CyclinD1 and c-Myc was obviously decreased compared with that of the control group(P<0.05). Conclusion:The growth of MCF-7 cells is significantly inhibited by pulchinenoside D, which may be involved in Wnt/β-catenin signaling pathway.

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