SHR大鼠肾脏去交感神经与主动脉miRNA相关性分析-东南大学学报医学版

SHR大鼠肾脏去交感神经与主动脉miRNA相关性分析

单位：遵义市第一人民医院心内科, 贵州遵义 563000

分类号：R363

出版年·卷·期（页码）：2018·37·第一期（6-11）

摘要：

目的：对自发性高血压大鼠（SHR）行肾脏去交感神经术（RSD），检测其降压效果以及血和主动脉组织中miRNAs，探讨SHR大鼠肾脏去交感神经与主动脉miRNA相关性。方法：SHR对照组，SHR手术组，正常大鼠（WKY）对照组和WKY手术组，20只/组，SHR对照组和WKY对照组均为假手术组，SHR手术组和WKY手术组接受RSD。记录所有大鼠术前和术后4、8、16和24周尾动脉血压，采用RT-PCR检测血清miRNAs及24周主动脉组织miRNAs（miR-150、miR-155、miR-124、miR-135a和miR-143/145）。结果：4组大鼠不同时间点的血压、miRNAs均有差别（P<0.05），与SHR对照组比，SHR手术组的收缩压、舒张压和miRNAs较低（P<0.05），同与WKY对照组比，WKY手术组的收缩压和舒张压较低（P<0.05），WKY对照和WKY手术组的miRNAs无统计学意义（P>0.05）。结论：RSD可降低SHR血压，可能与降低主动脉组织和循环血清miRNAs表达有关。

Objective: To study the effect of renal sympathetic denervation(RSD) for spontaneously hypertensive rats(SHR) and the relationship between RSD and expressions of miR-150、miR-155、miR-124、miR-135a and miR-143/145 in aorta artery tissue and peripheral blood. Method: A total of 40 healthy SHR Wistar rats and 40 normal rats(WKY) were divided randomly into operation and sham sub-groups,20 rats in each group: SHR control group, SHR operation group, WKY control group and WKY operation group, SHR control group and WKY control group accepted sham operation, SHR operation group and WKY operation group underwent operation. The miR-150, miR-155, miR-124, miR-135a and miR-143/145 in peripheral blood before and 4, 8, 16 and 24 weeks after operation,the values of systolic(SBP) and diastolic(DBP) blood pressure of rat tail artery;the above miRNAs in aorta artery tissue by RT-PCR after 24 weeks by killed rats. Results: The blood pressure and miRNAs of 4 groups were different at different time (P<0.05). Compared with the SHR control group, the systolic and diastolic blood pressure and miRNAs of SHR operation group were lower (P<0.05). Compared with the WKY control group, the systolic and diastolic blood pressure and miRNAs of WKY operation group were lower (P<0.05). The miRNAs of WKY control group and WKY operation group had no differences(P>0.05). Conclusion: It is effective and stable for RSD in SHR rats by lowerring miR-150, miR-155, miR-124, miR-135a and miR-143/145 in peripheral blood and aorta artery tissue.

参考文献：

[1] 车璐,杨宁,李拥军.经皮射频导管消融肾脏去交感神经支配术治疗顽固性高血压[J].中国普外基础与临床杂志,2013,20(3):340-343.
[2] 尚福军,王捷频,于亮,等.肾脏去交感神经对肾性高血压的影响及预测指标的筛选[J].岭南心血管病杂志,2015,21(6):858-861.
[3] VONGPATANASIN W.Resistant hypertension:a review of diagnosis and management[J].JAMA,2014,311(21):2216-2224.
[4] CHEN J H,WANG D Z,张玲玉,等.微小RNA对心血管系统发育、功能和高血压的调节作用[J].中华高血压杂志,2013,21(1):4-7.
[5] 黄颖,伍伟锋.自发性高血压大鼠肥厚左室心肌组织中微小RNA差别表达[J].中华高血压杂志,2010,18(1):81-84.
[6] YANG B,LIN H,XIAO J,et al.The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2[J].Nat Med,2007,13(5):486-491.
[7] 段练,熊兴江,王阶.MicroRNA与高血压病[J].中国中药杂志,2014,10(03):397-401.
[8] 许婵婵,韩卫青,肖冰,等.微小RNA在自发性高血压大鼠主动脉的差别表达[J].生理学报,2008,60(4):553-560.
[9] 唐青,邢晓为.miRNA在高血压疾病中的应用及调控机制[J].中华高血压杂志,2013,2(21):121-127.
[10] SCHLAICH M P,SOBOTKA P A,KRUM H,et al.Renal denervation as a therapeutic approach for hypertension:Novel implications for an old concept[J].Hypertension,2009,54(6):1195-1201.
[11] ARGACHA, J.F,P.VAN DE B P.Renal denervation:new treatment for refractory hypertension?[J].Rev Med Brux,2012,33(4):292-294.
[12] 段练,熊兴江,王阶.MicroRNA与高血压病[J].中国中药杂志,2014,39(3):397-401.
[13] 叶肖燕.高血压患者血清miR-124、NR3C2蛋白水平变化及意义[J].山东医药,2015,55(27):64-65.
[14] BOETTGER T,BEETZ N,KOSTIN S,et al.Acquistion of the contractive phenotype by murine arterial smooth muscle cells dependson on the Mir143/145gene cluster[J].J Clin Invest,2009,119(9):2634-2647.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录