姜黄素通过内质网应激信号通路诱导肝癌SMMC-7721细胞凋亡的作用研究-东南大学学报医学版

姜黄素通过内质网应激信号通路诱导肝癌SMMC-7721细胞凋亡的作用研究

单位：1. 江苏大学附属昆山医院肝胆外科, 江苏苏州 215300;
2. 江苏大学医学院, 江苏镇江 212013

分类号：R735.7

出版年·卷·期（页码）：2018·37·第一期（12-17）

摘要：

目的：检测姜黄素对肝癌SMMC-7721细胞增殖及凋亡的影响，并进一步探讨内质网应激反应在其中的作用。方法：应用2.5 μM、5 μM、10 μM、20 μM、50 μM的姜黄素处理肝癌SMMC-7721细胞，采用四甲基偶氮唑蓝（MTT）法检测细胞活性；Western Blot检测内质网应激相关蛋白GRP78、caspase-12、CHOP的表达；流式细胞术检测姜黄素对肝癌细胞凋亡的影响。结果：与对照组比较，2.5 μM、5 μM、10 μM、20 μM、50 μM的姜黄素对肝癌SMMC-7721细胞增殖均有抑制作用，并随作用时间的增加，增殖抑制作用更加显著（均P<0.05）；姜黄素能够诱导肝癌SMMC-7721细胞GRP78（P<0.05）、caspase-12（P<0.05）、CHOP（P<0.01）的表达，其表达量具有剂量依赖性；姜黄素能够诱导肝癌SMMC-7721细胞凋亡，与对照组比较（P<0.05），呈现出剂量依赖关系。结论：姜黄素通过内质网应激信号通路诱导肝癌细胞凋亡，抑制其增殖。

Objective: To investigate the effect of curcumin on ERS-related apoptosis and cell proliferation in smmc-7721 cells. Methods: SMMC-7721 cells were incubated with the blank vehicle, 2.5, 5, 10, 20 μM of curcumin. After intervention, cell proliferation were detected by MTT, protein expressions of GRP78, caspase-12, CHOP were assessed by western blotting. Cell apoptotic rate were detected by flow cytometry. Results: Compared with the control group, 2.5, 5, 10, 20 μM cucurmin inhibited the proliferation of hepatocellular carcinoma SMMC-7721 cells, and with the increase of the time, the inhibition of proliferation is more significant(All P<0.05). Curcumin could induce the expression of GRP78(P<0.05), caspase-12(P<0.05) and CHOP(P<0.01) in SMMC-7721 cells, and the expression of these proteins structures were dose dependent. Curcumin induced apoptosis of hepatocellular carcinoma SMMC-7721 cells, compared with the control group(P<0.05), showing a dose-dependent relationship. Conclusion: Curcumin may suppresse cell proliferation and induce apoptosis partly through activation of ERS signaling pathway in hepatocellular carcinoma cells.

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