炎症小体蛋白AIM2负调节抗RNA病毒反应发生-东南大学学报医学版

炎症小体蛋白AIM2负调节抗RNA病毒反应发生

单位：南京医科大学基础医学院, 江苏南京 211166

分类号：R392.1

出版年·卷·期（页码）：2018·37·第二期（220-225）

摘要：

目的：探索炎症小体蛋白AIM2在抗RNA病毒反应中的作用。方法：在野生型和AIM2基因敲除的巨噬细胞上感染不同RNA病毒，感染一定时间后用流式细胞仪检测病毒含量，用定量PCR和ELISA检测抗病毒基因和炎症因子表达，并用Western blot检测抗病毒信号通路蛋白的激活。结果：相对于野生型巨噬细胞，AIM2基因敲除细胞在RNA病毒感染后有更少的病毒存在，并且抗病毒效应分子在AIM2敲除细胞中表达明显升高，此外AIM2基因敲除可促进RNA抗病毒通路TBK1、IRF3和IKBα的激活。结论：炎症小体蛋白AIM2负调节抗RNA病毒反应。

Objective:To investigate the roles of AIM2 in anti-RNA viral response. Methods:WT and Aim2^-/- BMDMs were infected with various RNA virus and after infection infected cells (GFP⁺) were counted by FACS and the expressions of antiviral cytokines at gene and protein levels were detected by real-time PCR (q-PCR) and ELISA. Finally the activation of antiviral pathway was detected by using Western blot. Results:Compared to WT BMDM, Aim2^-/- cell had less RNA viral infection and higher antiviral cytokines production triggered by RNA virus. Consistently, the activation of TBK1, IRF3 and IKBα represented by phosphorylation was upregulated in Aim2 deficient cells in response to RNA viral infection. Conclusion:The results clearly demonstrate the negative role of AIM2 in anti-RNA viral response.

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