肝窦内皮细胞对肝细胞生物学特性影响的体外研究-东南大学学报医学版

肝窦内皮细胞对肝细胞生物学特性影响的体外研究

单位：1. 东莞东华医院肾内科, 广东东莞 523000;
2. 中山大学附属第五医院血液净化中心, 广东珠海 519000;
3. 华北理工大学基础学院病理生理系, 河北唐山 063009;
4. 中日友好医院临床医学研究所, 北京 100029;
5. 苏州瑞徕生物科技有限公司, 江苏苏州 215000;

关键词：永生化人肝细胞永生化人肝窦内皮细胞共培养肝功能衰竭

分类号：R318.14

出版年·卷·期（页码）：2018·37·第四期（566-572）

摘要：

目的：探讨永生化人肝窦内皮细胞（IHLESC）能否提高肝细胞的代谢、解毒及合成功能。方法：设立永生化人肝细胞（IHH）单独培养组和IHLESC与IHH共培养组，评价两种不同培养方式对肝细胞代谢、解毒和合成功能的影响；将两组细胞分别接种于微载体上，分别与肝衰竭患者血浆共孵育，比较两组IHH的解毒功能。结果：相比于单独培养组，共培养组IHH密切接触，聚集成团。共培养组IHH的谷氨酰胺合成酶（GS）、谷胱甘肽-S-转移酶（GST）、鸟苷二磷酸葡萄糖醛酸基转移酶1A1（UGT1A1）、白蛋白（ALB）表达量均显著高于单独培养组（P<0.01）。共培养组血浆总胆红素的下降及血浆尿素的上升均比单独培养组显著（P<0.01）。结论：用IHH与IHLSEC在微载体上共培养制备成新型生物人工肝的核心材料，可提高生物人工肝细胞的代谢、解毒及合成功能。

Objective:To investigate whether human liver sinusoidal endothelial cells can improve metabolism, detoxification and synthesis via co-culture of immortalized human hepatocytes(IHH)with immortalized human liver sinusoidal endothelial cells(IHLSEC). Methods:Two experimental groups were set up:IHH single culture, and IHLSEC and IHH co-culture groups. Metabolism, detoxification and synthesis in both cell groups were compared. Sequentially, the two groups cultured in micro-carriers were respectively incubated with plasma from patients with liver failure to compare detoxification function between the two groups. Results:Compared to the single culture group, IHH in the co-culture group had tight contact between cells, which were clumped together. The expression of GS, GST, UGT1A1, and ALB in the co-culture group was significantly higher than the other(P < 0.01). Co-culture group decreased plasma urea and increased plasma total bilirubin more significantly than the single group(P < 0.01). Conclusion:The new bioartificial liver, constituted of IHLSEC and IHH attached to micro-carriers, may have better metabolism, detoxification, synthesis, and functionality.

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