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microRNA-150在心房颤动患者心房组织中的表达及其对大鼠H9c2心肌细胞的影响
作者:李小兵  王军  吕瑛  张会军  黄建成  李红英  苏振宇 
单位:河北医科大学第一医院 心脏外科, 河北 石家庄 050031
关键词:microRNA-150 心房颤动 心肌细胞 胶原Ⅰ 基质金属蛋白酶-9 转化生长因子-β1 大鼠 
分类号:R-33;R541.75
出版年·卷·期(页码):2018·37·第四期(612-616)
摘要:

目的:探讨microRNA-150在心房颤动患者心房组织中的表达及其对大鼠H9c2心肌细胞的影响。方法:选择行风湿性心脏病瓣膜置换术心房颤动患者的心房组织(30例)作为心房颤动组,行风湿性心脏病瓣膜置换术窦性心律患者的心房组织(30例)作为对照组。将H9c2心肌细胞分为microRNA-150组(转染pGIPZ-miR-150慢病毒)、阴性对照组(转染pGIPZ慢病毒)和空白对照组(未转染病毒)。采用RT-PCR测定组织和细胞中microRNA-150表达和cMyb mRNA水平;采用Western blotting测定细胞中cMyb蛋白、基质金属蛋白酶-9(MMP-9)和转化生长因子-β1(TGF-β1)水平;采用ELISA法测定细胞中胶原Ⅰ水平。结果:心房颤动组心房组织中microRNA-150的相对表达量低于对照组(P<0.05)。microRNA-150组H9c2心肌细胞中microRNA-150表达量明显高于阴性对照组和空白对照组(P<0.05),cMyb mRNA和蛋白水平明显低于阴性对照组和空白对照组(P<0.05),胶原Ⅰ水平明显低于阴性对照组和空白对照组(P<0.05),MMP-9和TGF-β1水平明显低于阴性对照组和空白对照组(P<0.05);阴性对照组和空白对照组H9c2心肌细胞中各指标差异无统计学意义(P>0.05)。结论:心房颤动患者心房组织中microRNA-150表达量下降,上调心肌细胞中microRNA-150表达量可通过靶基因cMyb降低心肌细胞纤维化。

Objective:To investigate the expression of microRNA-150 in atrial fibrillation and its effect on rat H9c2 cardiomyocytes.Methods:30 cases of atrial tissue of patients with atrial fibrillation who underwent valve replacement were treated as atrial fibrillation group, and 30 cases of atrial tissue of patients with sinus thythun receiving valve replacement were selected as control group.H9c2 cardiomyocytes were divided into microRNA-150 group (transfected with pGIPZ-miR-150 lentivirus), negative control group (transfected with pGIPZ lentivirus) and blank control group (untransfected virus).The expression of microRNA-150 and cMyb mRNA in tissues and cells were determined by RT-PCR. The levels of cMyb protein, matrix metalloproteinase-9 (MMP-9) and transforming growth factor-β1 (TGF-β1) were measured by Western blotting. The level of collagen Ⅰ in cells was determined by ELISA. Results:The relative expression of microRNA-150 in the atrial tissue of the atrial fibrillation group was lower than that in the control group (P<0.05).The expression of microRNA-150 in H9c2 cardiomyocytes of the microRNA-150 group was significantly higher than that in the negative control group and the blank control group (P<0.05).The levels of cMyb mRNA and protein in H9c2 cardiomyocytes of the microRNA-150 group were significantly lower than those in the negative control group and the blank control group (P<0.05).The level of collagen Ⅰ in H9c2 cardiomyocytes of the microRNA-150 group was significantly lower than that of the negative control group and the blank control group (P<0.05).The levels of MMP-9 and TGF-β1 in H9c2 cardiomyocytes in the microRNA-150 group were significantly lower than those in the negative control group and the blank control group (P<0.05).There were no significant difference in each indicator of H9c2 cardiomyocytes between the negative control group and the blank control group (P> 0.05). Conclusion:The expression of microRNA-150 in atrial tissue of patients with atrial fibrillation decreases. Increasing the expression of microRNA-150 in cardiomyocytes can decrease myocardial cell fibrosis through the target gene cMyb.

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