Objective:To investigate the immunomodulatory effects of Chai Ge oral liquid on mice with bronchiolitis model. Methods:60 mice were divided into control group, model group, Chai Ge oral liquid low dose group (5.18 mg·kg-1·d-1), high dose group (23.1 mg·kg-1·d-1), ribavirin group (10 mg·kg-1·d-1), each group had 12 rats. Model establishment:after the mice were successfully anesthetized, 100 μl of RSV virus was instilled into the nasal cavity of the mice. After the nasal drops, the heads of the mice were kept back for 1 minute to allow the RSV virus fluid to flow into the bronchus and trachea of the mice once a day for 2 days.The lung tissue virus titer and lung index were detected.The expression of Helper T cells 17 (Th17) and Regulatory T (Treg) cells were detected by flow cytometry. The interleukin-4 (IL-4), interleukin-10 (IL-10),interleukin-17 (IL-17) and transforming growth factor-beta (TGF-β) were measured by enzyme-linked immunosorbent assay (ELISA). Results:The lung index, lung pathology score, Th17 cells, IL-4 and IL-17 levels in the low dose group, the high dose group and the ribavirin group were higher than those in the control group (P<0.05); the Treg cells, IL-10 and TGF-β levels were lower than those in the control group (P<0.05).The virus titer, lung index, lung pathology score, Th17 cell, IL-4 and IL-17 levels in the low dose group, the high dose group and the ribavirin group were lower than those in the model group (P<0.05); the Treg cells, IL-10 and TGF-β levels were higher than those in the model group (P<0.05).The virus titer, lung index, lung pathology score, Th17 cell, IL-4 and IL-17 levels in the high dose group were significantly lower than those in the low dose group and the ribavirin group (P<0.05); the Treg cells, IL-10 and TGF-β levels were higher than those in the low dose group and the ribavirin group (P<0.05). There was no significant difference in each indicators between the low dose group and the ribavirin group (P>0.05). Conclusion:Chai Ge oral solution may play a therapeutic role in mice with bronchiolitis model by immunoregulatory mechanism.
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