柴葛口服液对毛细支气管炎模型小鼠的免疫调节作用-东南大学学报医学版

柴葛口服液对毛细支气管炎模型小鼠的免疫调节作用

单位：1. 河南省南阳市中心医院儿科急诊, 河南南阳 473000;
2. 郑州儿童医院儿科, 河南郑州 450053

分类号：R562.21;R-332

出版年·卷·期（页码）：2018·37·第五期（792-796）

摘要：

目的：探讨柴葛口服液对毛细支气管炎模型小鼠的免疫调节作用。方法：将60只小鼠根据随机数字表法分为对照组、模型组、柴葛低剂量（5.18 mg·kg^-1·d^-1）组、柴葛高剂量（23.1 mg·kg^-1·d^-1）组、利巴韦林（10 mg·kg^-1·d^-1）组，每组12只。模型建立方法：小鼠麻醉成功后，顺小鼠鼻腔滴入呼吸道合胞病毒（RSV）病毒液100 μl，滴鼻后保持小鼠头部后仰1 min使RSV病毒液流入小鼠支气管、气管部位，1次·d^-1，共2 d。测定各组肺组织病毒滴度和肺指数，采用流式细胞术检测各组小鼠外周血辅助性T细胞17（Th17）和调节性T细胞（Treg）表达，采用酶联免疫吸附测定（ELISA）法测定各组小鼠血清白细胞介素-4（IL-4）、白细胞介素-10（IL-10）、白细胞介素-17（IL-17）、转化生长因子-β（TGF-β）水平。结果：模型组、柴葛低剂量组、柴葛高剂量组、利巴韦林组肺指数、肺病理学评分、Th17细胞表达和IL-4、IL-17水平均高于对照组（P<0.05），Treg细胞表达和IL-10、TGF-β水平低于对照组（P<0.05）；柴葛低剂量组、柴葛高剂量组、利巴韦林组病毒滴度、肺指数、肺病理学评分、Th17细胞表达和IL-4、IL-17水平均低于模型组（P<0.05），Treg细胞表达和IL-10、TGF-β水平高于模型组（P<0.05）；柴葛高剂量组病毒滴度、肺指数、肺病理学评分、Th17细胞表达和IL-4、IL-17水平低于柴葛低剂量组和利巴韦林组（P<0.05），Treg细胞表达和IL-10、TGF-β水平高于柴葛低剂量组和利巴韦林组（P<0.05）；柴葛低剂量组和利巴韦林组各指标差异无统计学意义（P>0.05）。结论：柴葛口服液可能通过免疫调节机制对毛细支气管炎模型小鼠发挥治疗作用。

Objective:To investigate the immunomodulatory effects of Chai Ge oral liquid on mice with bronchiolitis model. Methods:60 mice were divided into control group, model group, Chai Ge oral liquid low dose group (5.18 mg·kg^-1·d^-1), high dose group (23.1 mg·kg^-1·d^-1), ribavirin group (10 mg·kg^-1·d^-1), each group had 12 rats. Model establishment:after the mice were successfully anesthetized, 100 μl of RSV virus was instilled into the nasal cavity of the mice. After the nasal drops, the heads of the mice were kept back for 1 minute to allow the RSV virus fluid to flow into the bronchus and trachea of the mice once a day for 2 days.The lung tissue virus titer and lung index were detected.The expression of Helper T cells 17 (Th17) and Regulatory T (Treg) cells were detected by flow cytometry. The interleukin-4 (IL-4), interleukin-10 (IL-10),interleukin-17 (IL-17) and transforming growth factor-beta (TGF-β) were measured by enzyme-linked immunosorbent assay (ELISA). Results:The lung index, lung pathology score, Th17 cells, IL-4 and IL-17 levels in the low dose group, the high dose group and the ribavirin group were higher than those in the control group (P<0.05); the Treg cells, IL-10 and TGF-β levels were lower than those in the control group (P<0.05).The virus titer, lung index, lung pathology score, Th17 cell, IL-4 and IL-17 levels in the low dose group, the high dose group and the ribavirin group were lower than those in the model group (P<0.05); the Treg cells, IL-10 and TGF-β levels were higher than those in the model group (P<0.05).The virus titer, lung index, lung pathology score, Th17 cell, IL-4 and IL-17 levels in the high dose group were significantly lower than those in the low dose group and the ribavirin group (P<0.05); the Treg cells, IL-10 and TGF-β levels were higher than those in the low dose group and the ribavirin group (P<0.05). There was no significant difference in each indicators between the low dose group and the ribavirin group (P>0.05). Conclusion:Chai Ge oral solution may play a therapeutic role in mice with bronchiolitis model by immunoregulatory mechanism.

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