慢性疼痛对孕鼠及其子代认知功能的影响-东南大学学报医学版

慢性疼痛对孕鼠及其子代认知功能的影响

单位：1. 徐州医科大学护理学院, 江苏徐州 221004;
2. 徐州医科大学附属医院护理部, 江苏徐州 221002;
3. 北京清华长庚医院麻醉科, 北京 102218

分类号：R-332

出版年·卷·期（页码）：2018·37·第六期（985-989）

摘要：

目的：通过构建孕期小鼠慢性疼痛模型，观察慢性疼痛对孕鼠及子鼠认知功能的影响。方法：将交配成功的母鼠随机分为正常组、假手术组和慢性疼痛组，每组10只。慢性疼痛组母鼠足跖关节皮内注射无菌完全弗氏佐剂100 μl（即500 μg），诱导产生关节炎性痛。用水迷宫实验检测动物认知能力。结果：定位航行实验中慢性疼痛组孕鼠平均逃避潜伏期比假手术组长（P<0.001）。空间搜索实验中慢性疼痛组孕鼠穿越平台次数、平均游速、在原平台象限停留的时间比率及游程比率均低于假手术组（P<0.05）。子鼠出生后第3周，慢性疼痛组子鼠平均逃避潜伏期比假手术组长（P<0.01），穿越平台次数少于假手术组子鼠（P<0.05），平均游速、在原平台象限停留的时间比率和游程比率与假手术组子鼠比较，差异无统计学意义（P>0.05）。结论：孕期慢性疼痛会导致母体认知能力的下降，也会影响其子代幼年期的认知功能。

Objective:To observe the effects of chronic pain on the cognitive function of pregnant mice and their offspring by constructing the animal model of chronic pain in pregnant mice. Methods:The pregnant mice were divided into normal group, sham-operation group and chronic pain group (10 in each group). Aseptic complete Freund's adjuvant 100 μl (500 μg) was injected intracutaneously in the paw joint of pregnant mice to induce arthritis pain. Water labyrinth experiment was performed to evaluate the cognitive ability of the animals. Results:In the place navigation test, the average escape latency of the mice in chronic pain group were longer than that in sham operation group (P<0.001).In the spatial search experiment, the number of times of crossing the platform, the average swimming speed, the swimming time ratio and the swimming distance ratio staying in the original platform quadrant of the mice in the chronic pain group were less than those in the sham operation group (all P<0.05).At the third week after their offspring birth, the average escape latency of offspring mice in the chronic pain group was longer than that in the sham operation group(P<0.01), and the number of times of crossing the platform was less than that in the sham operation group (P<0.05).There was no difference in the average swimming speed, the swimming time ratio and the swimming distance ratio staying in the original platform quadrant between the offspring mice of the two groups (P>0.05). Conclusions:Chronic pain during pregnancy can lead to a decline in maternal cognitive ability and also affect the cognitive function of the offspring in infancy.

参考文献：

[1] International Pain Summit of The International Association for the Study of Pain.Declaration of Montréal:declaration that access to pain management is a fundamental human right[J].J Pain Palliat Care Pharmacother,2011,25(1):29-31.
[2] DOLEYS DM.How neuroimaging studies have challenged us to rethink:is chronic pain a disease?[J].J Pain,2010,11(4):399-400.
[3] 周芳,陈翠,谢娜,等.慢性疼痛与妊娠期妇女焦虑、抑郁的相关性研究[J].中国疼痛医学,2014,20(11):840-842.
[4] ZHOU F,CHEN C,HUANG Y,et al.The effects of enriched environmental intervention on the parturition outcome and filial health of pregnant mice with chronic pain[J].Biol Res Nurs,2018,20(3):264-271.
[5] BERRYMAN C,STANTON T R,JANE BOWERING K,et al.Evidence for working memory deficits in chronic pain:a systematic review and meta-analysis[J].Pain,2013,154(8):1181-1196.
[6] WILLIAMS A C,CRAIG K D.Updating the definition of pain[J].Pain,2016,157(11):2420-2423.
[7] BAKER K S,GIBSON S,GEORGIOU-KARISTIANIS N,et al.Everyday executive functioning in chronic pain:specific deficits in working memory and emotion control,predicted by mood,medications,and pain interference[J].Clin J Pain,2016,32(8):673-680.
[8] 李丽,陈翠,周芳,等.慢性疼痛对孕鼠及其子代抑郁行为的影响[J].东南大学学报(医学版),2016,35(6):938-942.
[9] Van RYCKEGHEM D M,Van DAMME S,ECCLESTON C,et al.The efficacy of attentional distraction and sensory monitoring in chronic pain patients:a meta-analysis[J].Clin Psychol Rev,2018,59:16-29.
[10] PRAKASH S,GOLWALA P.Phantom headache:pain-memory-emotion hypothesis for chronic daily headache?[J].J Headache Pain,2011,12(3):281-286.
[11] OZAWA T,YCU E A,KUMAR A,et al.A feedback neural circuit for calibrating aversive memory strength[J].Nat Neurosci,2017,20(1):90-97.
[12] 王瑞,樊欣鑫.不同吸入浓度及吸入时间的七氟烷对生长发育期大鼠认知功能的影响[J].现代医学,2016,44(1):15-19.
[13] 侯永恒.表观遗传调控对骨癌痛大鼠痛觉过敏与认知障碍以及BDNF介导的突触可塑性的影响[D].苏州:苏州大学,2016.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录