叶黄素对H<sub>2</sub>O<sub>2</sub>作用下MIO-M1细胞活性及迁移的影响-东南大学学报医学版

叶黄素对H₂O₂作用下MIO-M1细胞活性及迁移的影响

单位：大连医科大学附属第一医院眼科, 辽宁大连 116011

分类号：R774.5

出版年·卷·期（页码）：2018·37·第六期（989-992）

摘要：

目的：探讨不同浓度叶黄素对过氧化氢（H₂O₂）作用下人眼Müller细胞系MIO-M1细胞活性及迁移的影响。方法：将MIO-M1细胞随机分为对照组、H₂O₂损伤组及叶黄素+H₂O₂共处理组，采用四甲基偶氮唑盐比色法（MTT法）及改良细胞划痕法检测不同浓度H₂O₂及加入叶黄素共处理后MIO-M1细胞活性及迁移率。结果：MTT法显示，与对照组相比，H₂O₂损伤组MIO-M1细胞活性显著抑制；与H₂O₂损伤组相比，加入叶黄素共处理后可增加MIO-M1细胞活性，差异均有统计学意义（P<0.05）。改良细胞划痕法表明，与对照组相比，H₂O₂损伤组MIO-M1细胞迁移率显著下降；与H₂O₂损伤组相比，加入叶黄素共处理后可提高MIO-M1细胞迁移率，差异均有统计学意义（P<0.05）。结论：叶黄素对H₂O₂作用下MIO-M1细胞活性及迁移具有保护作用。

Objective:To investigate the effect of different concentrations of lutein on viability and migration of human Müller cell line MIO-M1cells under hydrogen peroxide (H₂O₂) induced oxidative stress. Methods:MIO-M1 cells were randomly divided into control group, H₂O₂ group and co-treatment(lutein+H₂O₂) group. The viability and migration of MIO-M1 cells which were treated with different concentrations of H₂O₂ and co-treatment with lutein were analyzed by MTT assay and modified wound scratch assay.Results:MTT assay showed that the viability of MIO-M1 cells was significantly decreased in the H₂O₂ group compared with the control group. The viability of MIO-M1 cells in co-treatment group were significantly increased compared with the H₂O₂ group(all P<0.05).Modified wound scratch assay indicated that the migration rate of MIO-M1 cells was significantly decreased in the H₂O₂ group compared with control group. The migration rate of MIO-M1 cells in co-treatment group was significantly increased compared with H₂O₂ group(all P<0.05). Conclusion:Lutein can protect the viability and migration of MIO-M1cells from H₂O₂-induced oxidative stress.

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