>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
改良多重实时荧光定量PCR法测定抑癌基因MGMT、p16、CDH13和RASSF1A甲基化在肺癌早期诊断中的作用
作者:钟云华  李燕 
单位:云南省第一人民医院/昆明理工大学附属医院 老年医学科, 云南 昆明 650032
关键词:改良多重实时荧光定量PCR法 抑癌基因 MGMT p16 CDH13 RASSF1A 肺癌早期诊断 
分类号:734.2
出版年·卷·期(页码):2019·38·第一期(33-38)
摘要:

目的:建立改良多重实时荧光定量PCR法,检测抑癌基因MGMR、p16、CDH13和RASSF1A基因甲基化,探讨其在肺癌早期诊断中的应用。方法:选取30例病理确诊为肺癌的患者作为肺癌组,30例病理确诊为良性病变的患者作为对照组。建立改良实时荧光定量PCR体系。检测受试者血清MGMT、p16、CDH13和RASSF1A基因甲基化,对比两组基因甲基化阳性率,计算四项基因甲基化单独及联合用于肺癌早期诊断的敏感度和特异度。结果:肺癌组受试者血清MGMT、p16、CDH13和RASSF1A显著高于对照组(P<0.05);MGMT、p16、CDH13和RASSF1A四项基因甲基化联合检测用于肺癌早期诊断的敏感度和特异度显著高于单项指标检测(P<0.05)。结论:建立了多重实时荧光定量PCR法用于MGMT、p16、CDH13和RASSF1A基因甲基化检测,联合检测用于肺癌早期诊断具有较高的敏感度和特异度。

Objective:A modified multiplex real-time fluorescence quantitative PCR method was established to detect the methylation of tumor suppressor genes MGMR, p16, CDH13 and RASSF1A, and to explore its application in early diagnosis of lung cancer. Methods:Thirty patients with lung cancer were confirmed by pathology and selected as lung cancer group and 30 patients with benign disease confirmed by pathology as control group. Establish a modified real-time fluorescence quantitative PCR system. Methylation of MGMT, p16, CDH13, and RASSF1A genes was detected in the subjects, and the positive rate of methylation was compared between the two groups. The sensitivity and specificity of the gene methylation alone and in combination for the early diagnosis of lung cancer were calculated. Results:Serum MGMT, p16, CDH13, and RASSF1A levels were significantly higher in lung cancer group than in the control group (P<0.05). The sensitivity and specificity of MGMT, p16, CDH13, and RASSF1A genes for the early diagnosis of lung cancer were significantly higher than those diagnosed separately (P<0.05). Conclusion:The established multiplex real-time fluorescence quantitative PCR method has been used to detect the methylation of MGMT, p16, CDH13 and RASSF1A genes. Which has high sensitivity and specificity for the early diagnosis of lung cancer.

参考文献:

[1] FITZMAURICE C,DICKER D,PAIN A.The Global burden of cancer 2013[J].JAMA Oncol,2015,1(4):505-527.
[2] GBD 2013 MORTALITY AND CAUSES OF DEATH COLLABORATORS.Global,regional,and national age-sex specific all-cause and cause-specific mortality for 240 causes of death,1990-2013:A systematic analysis for the global burden of disease study 2013[J].Lancet,2015,385(9963):117-171.
[3] 杜立平,秦晓玲.非小细胞肺癌患者p53蛋白和MMP2表达水平与同步放化疗的相关性[J].标记免疫分析与临床,2017,24(9):1015-1017.
[4] YUAN S,YU Z,LIU Q,et al.GPC5,a novel epigenetically silenced tumor suppressor,inhibits tumor growth by suppressing Wnt/β-catenin signaling in lung adenocarcinoma[J].Oncogene,2016,35(47):6120-6131.
[5] CHUNG J H,LEE H J,KIM B H,et al.DNA methylation profile during multistage progression of pulmonary adenocarcinomas[J].Virchows Arch,2011,459(2):201-211.
[6] ZHANG Y,WANG R,SONG H,et al.Methylation of multiple genes as a candidate biomarker in non-small cell lung cancer[J].Cancer Lett,2011,303(1):21-28.
[7] 左江华,李宗良,任宏涛.血清CEA、CA125、CA199联合检测在肺癌诊断中的应用价值[J].标记免疫分析与临床,2016,23(06):668-671.
[8] RAJABI H,TAGDE A,ALAM M,et al.DNA methylation by DNMT1 and DNMT3b methyltransferases is driven by the MUC1-Concoprotein in human carcinoma cells[J].Oncogene,2016,35(50):6439-6445.
[9] BAYLIN S B, JONES P A.Epigenetic determinants of cancer[J].Cold Spring Harb Perspect Biol,2016,8(9):18.
[10] 刘晓辉.晚期非小细胞肺癌原发灶和转移灶ROS1融合基因表达差异分析[J].实用癌症杂志,2017,32(6):891-893.
[11] 杨洁,袁江伟,王玉祥,等.MGMT基因甲基化水平与恶性胶质瘤细胞对烷化剂耐药性的相关性[J].中国老年学杂志,2015,35(5):1949-1950.
[12] 时洁,耿晓康.盐酸吉西他滨对非小细胞肺癌患者骨髓CD34+造血细胞的毒性研究[J].实用癌症杂志,2017,32(11):1797-1800.
[13] 张亚龙,房念珍,尤嘉琮,等.肿瘤细胞代谢与肿瘤转移相互关系的研究进展[J].中国肺癌杂志,2014,17(11):812-818.
[14] 卢兴兵,李勤,石佳,等.多种血清肿瘤标志物联合检测诊断非小细胞肺癌的临床价值[J].现代医学,2017,45(10):1417-1421.
[15] 程斌,钟里科,王增,等.从肺癌组织提取高纯度总RNA方法的优化及应用[J].中国现代应用药学,2016,33(5):539-543.
[16] 邸红芹, 杨永辉, 朱桂云,等. 改良多重实时荧光定量PCR检测流感A型和B型病毒[J]. 河北医药, 2016, 38(23):3529-3532.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 405977 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-83272481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364