Objective: To observe the effects of Ophiopogon japonicuson polysaccharide on serum superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT) and malondialdehyde (MDA) in diabetic peri-menopausal rats, and explore its mechanism of action. Methods: The type 2 diabetes premenopausal rats were established by combined removal of bilateral ovaries with streptozotocin induction after giving high sugar and high fat feed for 8 weeks. Fifty model rats were randomly divided into model group,positive control group(melbine 0.25 g·kg-1 was given 10 days after molding) and Ophiopogon japonicuson polysaccharide low, medium and high dose group (100, 200, 400 mg·kg-1 were given respectively 10 days after molding), 10 in each group. Another 10 rats were selected as the sham operation group. The levels of body weight, blood glucose, serum SOD, GSH-Px, CAT and MDA in each group were compared before and after treatment. Levels of serum estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in each group were detected by ELISA and compared each other. Results: Compared with the model group, the body weight of the rats in the positive control group and low,medium, high dose polysaccharide groups were increased and blood glucose levels were decreased significantly (all P < 0.05).The levels of serum SOD, GSH-Px and CAT were significantly increased (all P < 0.05), and serum MDA were significantly decreased (P < 0.05); serum E2 was significantly increased (P < 0.05), serum FSH and LH were significantly decreased (all P < 0.05). Conclusion: Ophiopogon japonicuson polysaccharide can protect type 2 diabetes perimenopenesis, and its mechanism may be related to improving antioxidant capacity and regulating estrogen levels. |
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