微小RNA-139-5p对AS患者血管平滑肌细胞增殖和迁移作用的研究-东南大学学报医学版

微小RNA-139-5p对AS患者血管平滑肌细胞增殖和迁移作用的研究

单位：1. 青岛大学附属医院神经内科, 山东青岛 266000;
2. 诸城市人民医院神经内科, 山东潍坊 262200

分类号：R543;Q522

出版年·卷·期（页码）：2020·39·第四期（447-451）

摘要：

目的：检测微小RNA-139-5p（miR-139-5p）在动脉粥样硬化（AS）患者中的表达情况，分析miR-139-5p的临床价值及对人血管平滑肌细胞（HVSMCs）生物学功能的调控作用。方法：采用荧光定量PCR技术检测112例AS患者（AS组）和78例健康志愿者（对照组）血清miR-139-5p的表达情况，用受试者工作特征曲线（ROC曲线）评估miR-139-5p对AS的诊断价值。采用CCK-8实验和Transwell实验分析miR-139-5p对HVSMCs增殖和迁移能力的影响。结果：与对照组相比，AS组患者miR-139-5p的表达明显下调（P<0.001），同时AS组患者血清miR-139-5p的表达水平与颈动脉内中膜厚度（CIMT）呈负相关（r=-0.757 9，P<0.001）。miR-139-5p诊断AS的ROC曲线下面积（AUC）为0.888，具有较高敏感性（86.6%）和特异性（84.6%）。miR-139-5p低表达会促进HVSMCs的增殖和迁移能力，相反miR-139-5p过表达会显著地抑制HVSMCs的增殖和迁移能力（P<0.001）。结论：miR-139-5p的低表达可能是AS的潜在诊断标志物，过表达miR-139-5p可能通过抑制HVSMCs的增殖和迁移发挥改善AS的潜能。

Objective: To assess the clinical value of MicroRNA-139-5p (miR-139-5p) and analyze its regulatory effect on the biological behavior of vascular smooth cells (HVSMCs) by detecting the expression of miR-139-5p in atherosclerosis (AS) patients. Methods: The expression of serum miR-139-5p in 112 AS patients(AS group) and 78 healthy controls (control group) were detected by fluorescence quantitative PCR. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of miR-139-5p in AS. The effect of miR-139-5p on HVSMCs proliferation and migration was analyzed by CCK-8 assay and Transwell assay. Results: The expression of miR-139-5p in AS group was significantly down-regulated compared with the healthy control group (P<0.001). Meanwhile, the expression level of miR-139-5p in serum of AS group was negatively correlated with carotid intima-media thickness (CIMT) (r=0.757 9, P<0.001). The area under the ROC curve (AUC) was 0.888, with a high sensitivity (86.6%) and specificity (84.6%). In addition, the low expression of miR-139-5p promoted the proliferation and migration of HVSMCs, while the overexpression of miR-139-5p significantly inhibited the proliferation and migration of HVSMCs (P<0.001). Conclusion: The decreased expression of miR-139-5p may be a potential diagnostic marker of AS, and overexpression of miR-139-5p may improve AS by inhibiting the proliferation and migration of HVSMCs.

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