小脑锌指蛋白5与肝细胞癌临床病理特征及预后的关系-东南大学学报医学版

小脑锌指蛋白5与肝细胞癌临床病理特征及预后的关系

单位：1. 应城市人民医院病理科, 湖北应城 432400;
2. 应城市人民医院肝胆外科, 湖北应城 432400

分类号：R735.7

出版年·卷·期（页码）：2021·40·第四期（494-499）

摘要：

目的：探讨小脑锌指蛋白5（ZIC5）与肝细胞癌（简称肝癌）临床病理特征及预后的关系。方法：选取我院2010年1月至2013年1月手术切除的94例肝癌组织标本及与其配对的癌旁组织标本。随机选取其中6例标本用蛋白免疫印迹法检测组织中ZIC5的表达。用免疫组化染色法检测所有组织中ZIC5的表达，根据染色结果将患者分为高表达组和低表达组，比较两组患者的生存预后。生存分析采用Kaplan-Meier法，多因素分析采用COX比例风险回归模型。结果：肝癌组织ZIC5相对表达量高于癌旁组织（0.86±0.13 vs 0.25±0.08，P<0.05），且肝癌组织中ZIC5高表达率为62.77%（59/94），ZIC5表达水平与肝癌分化程度、临床分期、远处转移及肝硬化有关（均P<0.05）。肝癌分化程度、临床分期、远处转移及ZIC5高表达是肝癌患者总生存期和无瘤生存期的独立影响因素（均P<0.05），高表达组总生存率和无瘤生存率均低于低表达组（均P<0.05）。结论：肝癌组织中ZIC5表达水平普遍升高，并且与肝癌进展及肝硬化有关，ZIC5高表达可能预示患者生存预后不良。

Objective: To investigate the relationship between the zinc finger protein of the cerebellum 5(ZIC5) and the clinicopathological features and prognosis of hepatocellular carcinoma(HCC). Methods: From January 2010 to January 2013, 94 cases of HCC tissue samples and matched adjacent tissue samples were selected. The expressions of ZIC5 in 6 randomly selected samples were detected by Western blot. Immunohistochemical staining was used to determine the expressions of ZIC5 in all tissues. According to the staining results, the patients were divided into high expression group and low expression group. Kaplan-Meier method and multivariate COX proportional hazard regression model were employed for survival analysis. Results: The relative expression of ZIC5 in hepatocellular carcinoma tissues was higher than that in adjacent tissues(0.86±0.13 vs 0.25±0.08, P<0.05). And the high expression rate of ZIC5 in tumor tissues was 62.77%(59/94), which was related to the degree of differentiation,clinical stage, distant metastasis and cirrhosis (all P<0.05). The degree of differentiation, clinical stage, distant metastasis and high expression of ZIC5 were the independent influencing factors of overall survival and tumor free survival(P<0.05). The overall survival and free-tumor survival rate in high expression group were lower than those in low expression group(P<0.05). Conclusion: The expression level of ZIC5 in HCC is generally elevated, which is associated with the progression of HCC and liver cirrhosis. The high expression of ZIC5 may indicate the poor prognosis in patients with HCC.

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