Objective: To investigate the expression and significance of cell adhesion molecule Ninjurin2 in gliomas. Methods: Immunohistochemical staining was used to detect the expression levels of Ninjurin2 in 88 glioma tissues and 30 normal brain tissues. COX multivariate analysis and Kaplan-Meier curve were adopted to analyze the survival prognosis of glioma patients. Western-blotting assay was used to determine the expression of Ninjurin2 in human glioma cell lines U251, U87, U373 and normal glial cell lines SVG. U251 cells were transferred into Ninjurin2 siRNA sequence(Ninjurin2-siRNA group) and negative control siRNA sequence(NC-siRNA group) with Lipotectamine respectively. Cell proliferation assay was used to evaluate the ability of cell proliferation in each group. The invasion and migration of cancer cells were detected by Transwell experiment in different groups. Results: The expression level of Ninjurin2 protein in glioma tissue was 65.91%(58/88), which was 20.00%(6/30) higher than that in normal brain tissue(P<0.05). COX multivariate analysis indicated that the preoperative Karnofsky score(OR=2.312), WHO classification(OR=2.008), chemotherapy(OR=1.600) and Ninjurin2(OR=2.816) were independent factors affecting the overall survival of glioma patients(P<0.05). Kaplan-Meier survival analysis results indicated that the median survival time of Ninjurin2 high expression group was 11 months, which was significantly lower than 19 months of low expression group(P=0.004). Compared with SVG cells, the expression of Ninjurin2 protein in U251, U87 and U373 cells was higher(all P<0.05). Compared with the NC-siRNA group, the proliferation and invasion ability of U251 cells in the Ninjurin2-siRNA group was significantly decreased(P<0.05). Conclusion: Ninjurin2 involves in the progression of glioma, and high expression of Ninjurin2 is associated with the poor survival prognosis of the patients. |
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