Objective: To explore the effect of baicalin's regulation of PI3K/AKT pathway on lipopolysaccharid (LPS) induced abortion uterine macrophages in rats. Methods: 60 rats were divided into control group, LPS group, LPS+baicalin group, and LPS+baicalin+LY294002 group (n=15). A miscarriage model was induced by LPS injection through the tail vein. Intervention was performed by injecting baicalin through the tail vein. The PI3K/AKT pathway was inhibited by intraperitoneal injection of LY294002 (1 mg·kg-1). On the 10th day of pregnancy, the rats were euthanized to observe the embryonic development. The abortion rate and embryo absorption rate were calculated. The levels of serum inflammatory cytokines and uterine macrophages were detected by ELISA and flow cytometry. Immunohistochemical staining was used to detect the level of macrophage M1 polarization marker protein CD86 and M2 polarization marker protein CD206, so as to analyze the polarization of macrophages in decidua tissue. The transcription and translation levels of PI3K and AKT were detected by RT-qPCR and Western blotting. Results: The differences in the indicators of the four groups were statistically significant (all P<0.05). The embryo absorption of the LPS+baicalin group were 6 cases, and the abortion were 5 cases, which were significantly lower than those of the LPS group(15 cases, all P<0.05). The proportion of TNF-α[(397.53±48.27) pg·ml-1], IL-1β[(61.28±8.34) pg·ml-1] and uterine macrophages in the LPS group were significantly higher than those in the control group (all P<0.05). The proportion of TNF-α[(214.86±25.14) pg·ml-1], IL-1β[(49.74±6.79) pg·ml-1] and uterine macrophages in the LPS+baicalin group were significantly lower than those in the LPS group (all P<0.05). The proportion of M1 cells in the LPS group was significantly higher than that in the control group, while the proportion of M2 cells and the expression levels of PI3K and AKT mRNA were significantly lower than those in the control group (all P<0.05). The proportion of M1 cells in the LPS+baicalin group was significantly lower than that in the control group, while the proportion of M2 cells and the expression levels of PI3K and AKT mRNA were significantly higher than those in the control group (all P<0.05). The embryo absorption(14 cases) and abortion(13 cases) of the LPS+baicalin+LY294002 group were significantly higher than those of the LPS+baicalin group (all P<0.05). The proportion of TNF-α[(362.75±40.29) pg·ml-1], IL-1β[(57.62±7.67) pg·ml-1], macrophages, the proportion of M1 type cells in the LPS+baicalin+LY294002 group were significantly higher than those in the LPS+baicalin group, M2 type The cell ratio, PI3K and AKT protein levels were significantly lower than those in the LPS+baicalin group (P<0.05). Conclusion: Baicalin may promote the PI3K/AKT pathway to reduce the proportion of uterine macrophages and induce uterine macrophages to polarize to M2 and inhibit M1 thus to inhibit abortion. |
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