Objective: To explore the protective effect of Fagopyrum dibotrys on the animal model of ulcerative colitis(UC) and the regulation of IL-6/STAT3 transduction pathway. Methods: Forty-five mice were divided into control group, UC group, and UC+Fagopyrum dibotrys group(n=15). Use 3% dextransulfate sodium salt(DSS) was applied to induce UC model. Fagopyrum dibotrys was intervened by gavage. The colon length, disease activity index, intestinal mucosal permeability, inflammatory cytokine levels in tissues, tight junction protein expression levels, IL-6 and STAT3 transcription and protein expression levels were detected. Results: The disease activity index, IL-1β, TNF-α, intestinal mucosal permeability, IL-6 and STAT3 mRNA and protein levels in the UC group were significantly higher than those in the control group, ZO-1 and Occludin protein, and the colon length were significantly lower than those in the control Group(P<0.05). The disease activity index, IL-1β, TNF-α, intestinal mucosal permeability, IL-6 and STAT3 mRNA and protein levels in the UC+Fagopyrum dibotrys group were significantly lower than those in the UC group, ZO-1 and Occludin protein, and the colon length were significantly higher than those in the UC group(P<0.05). Conclusion: Fagopyrum dibotrys can inhibit IL-6/STAT3 pathway to inhibit inflammatory response, protect the intestinal mucosal barrier and relieve UC. |
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