Objective: To construct a three-dimensional biomimetic platform by using photonic crystal microcarriers for tumor cell culture, and the drug screening effect of this platform was preliminarily tested. And also the chemotherapeutic effect of the HMSN compound drug delivery system,which established by our laboratory, on CD117+ CD44+ A2780 and A2780 cells in this three-dimensional culture environment was preliminarily studied. Methods: CD117+ CD44+ A2780 and A2780 cells were respectively inoculated on conventional and three-dimensional bionic platforms, then DOX, PTX, CDDP and 5-FU were added,the inhibition rate and apoptosis rate of each group were calculated respectively to verify the three-dimensional applicability of bionic platforms. HMSN compound drug delivery system (loaded with DOX) were added to each group, the same dose of free drug was added to the control group, the apoptotic rate of each group was detected after co-incubating for 12 hours. Results: The chemotherapeutic effects of DOX, PTX, CDDP and 5-FU on CD117+ CD44+ A2780 and A2780 cells in the three-dimensional culture platform were significantly decreased (P<0.05), which indicated that the photonic crystal microcarriers could provide a biomimetic three-dimensional growth environment for the tumor cells. The pro-apoptotic effects of the same amount of free drugs on cultured cells in three-dimensional and conventional environments were significantly lower than those in the HMSN compound drug delivery system (P<0.05), prove that the HMSN compound drug delivery system may have better in vivo effects. Conclusion: The photonic crystal microcarriers constructed in this study can provide a biomimetic three-dimensional environment for the growth of tumor cells, which can be used for the preliminary screening of chemotherapeutic drugs, the HMSN compound drug delivery system exhibits a good antitumor effect on ovarian cancer cells in the three-dimensional culture environment, which provides strong basis for future animal and in vivo experiments. |
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