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MICAL2、miR-340-5p在胃癌组织中的表达及其临床意义
作者:段战锋1  张巍巍2  申晓燕1 
单位:1. 河南省许昌市中心医院鹿鸣湖院区 肿瘤内科二病区 河南 许昌 461000;
2. 河南省许昌市中心医院鹿鸣湖院区 胃肠外科, 河南 许昌 461000
关键词:胃癌 MICAL2 miR-340-5p 相关性 预后 
分类号:R735.2
出版年·卷·期(页码):2023·42·第二期(227-233)
摘要:

目的:探讨MICAL2、miR-340-5p在胃癌组织中的表达及其临床意义。方法:选取2017年1月至2018年6月在我院进行胃癌根治术的96例胃癌患者为研究对象,收集患者的胃癌组织及离肿瘤边缘5 cm处的癌旁组织。采用免疫组织化学染色法观察胃癌组织及癌旁组织中MICAL2的表达情况;Western Blot法检测胃癌组织中MICAL2蛋白水平,实时荧光定量PCR(qRT-PCR)法检测胃癌组织中miR-340-5p表达水平。分析MICAL2、miR-340-5p表达水平与患者临床病理参数的关系;Pearson法分析MICAL2与miR-340-5p表达的相关性;对患者随访3年,利用Kaplan-Meier法分析不同MICAL2、miR-340-5p水平与患者3年累积生存率的关系;采用多因素Cox回归风险模型分析胃癌患者的预后影响因素。结果:胃癌患者癌组织中MICAL2、miR-340-5p表达水平均明显高于癌旁组织(P<0.05);胃癌组织中MICAL2蛋白阳性表达率为71.88%(69/96),癌旁组织中MICAL2蛋白阳性表达率为11.46%(11/96)(P<0.05);胃癌组织中MICAL2蛋白和miR-340-5p表达与淋巴结转移、TNM分期有关(P<0.05);胃癌组织中MICAL2与miR-340-5p的表达水平呈正相关(r=0.313,P<0.05);Kaplan-Meier分析结果显示,miR-340-5p高表达组患者3年累积生存率显著低于低表达组(Log rank χ2=9.096,P=0.003),MICAL2蛋白高表达组患者3年累积生存率显著低于低表达组(Log rank χ2=4.329,P=0.037);Cox回归分析结果显示,MICAL2、miR-340-5p、淋巴结转移、TNM分期均是影响患者预后的危险因素(P<0.05)。结论:MICAL2、miR-340-5p在胃癌组织中明显高表达,二者与胃癌患者病情发展和预后密切相关,可能成为胃癌基因治疗的新靶点。

Objective: To investigate the expression and clinical significance of MICAL2 and miR-340-5p in gastric cancer tissues. Methods: Ninety-six patients with gastric cancer who came to our hospital for radical gastric cancer surgery from January 2017 to June 2018 were selected as the research objects. The gastric cancer tissues of the surgical patients and the adjacent tissues 5 cm from the tumor edge were collected. MICAL2 expression in gastric cancer tissues and adjacent tissues was detected by immunohistochemical staining; Western Blot method was used to detect the level of MICAL2 protein in gastric cancer tissues, real-time fluorescent quantitative PCR(qRT-PCR) method was used to detect the expression level of miR-340-5p in gastric cancer tissues, the relationship between the expression levels of MICAL2 and miR-340-5p and the clinical pathological parameters of patients was analyzed; Pearson method was used to analyze the correlation between MICAL2 and miR-340-5p expression; the patients were followed up for 3 years, and the Kaplan-Meier method was used to analyze the relationship between different levels of MICAL2 and miR-340-5p and the 3-year cumulative survival rate of patients. Multivariate Cox regression risk model was used to analyze the prognostic factors of gastric cancer patients. Results: The expression levels of MICAL2 and miR-340-5p in the cancer tissues of patients with gastric cancer were significantly higher than those in the adjacent tissues(P<0.05); the positive expression rate of MICAL2 protein in gastric cancer tissue was 71.88%(69/96), and the positive expression rate of MICAL2 protein in adjacent tissues was 11.46%(11/96)(P<0.05).The expressions of MICAL2 protein and miR-340-5p in gastric cancer were correlated with lymph node metastasis and TNM staging(P<0.05); the expression levels of MICAL2 and miR-340-5p in gastric cancer tissues were positively correlated(r=0.313, P<0.05). Kaplan-Meier analysis showed that the 3-year cumulative survival rate of patients in the high expression group of miR-340-5p was significantly lower than that in the low expression group(Log rank χ2=9.096, P=0.003), the 3-year cumulative survival rate of patients in the high expression group of MICAL2 protein was significantly lower than that in the low expression group(Log rank χ2=4.329, P=0.037). The results of Cox regression analysis showed that MICAL2, miR-340-5p, lymph node metastasis and TNM stage were all risk factors affecting the prognosis of patients(P<0.05). Conclusion: MICAL2 and miR-340-5p are significantly overexpresed in gastric cancer tissues, which are closely related to the development and prognosis of gastric cancer, and may become new targets for gene therapy of gastric cancer.

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