circMyt1l/rno-let-7d-5p/BDNF在少突胶质细胞前体细胞中的作用研究-东南大学学报医学版

circMyt1l/rno-let-7d-5p/BDNF在少突胶质细胞前体细胞中的作用研究

单位：1. 江苏卫生健康职业学院, 江苏南京 211800;
2. 扬州大学附属医院, 江苏扬州 225012;
3. 东南大学附属中大医院, 江苏南京 210009;
4. 东南大学医学院, 江苏南京 210009

分类号：R-33; R742

出版年·卷·期（页码）：2023·42·第三期（451-456）

摘要：

目的: 探讨circMyt1l/rno-let-7d-5p/脑源性神经营养因子(BDNF)在少突胶质细胞前体细胞(OPCs)中的表达变化和相互作用。方法: 运用生物信息学分析方法,分析脑室周围白质损伤幼鼠脑组织中具有差异表达的circMyt1l,和其相互作用的miRNA和mRNA;分离培养出OPCs,将其分为对照组(Mock组)和氧糖剥夺(OGD)1、2、3 h组,即OGD1、OGD2、OGD3组,采用qRT-PCR法检测circMyt1l、rno-let-7d-5p基因表达水平,使用蛋白质印迹法检测BDNF蛋白表达水平。结果: 生物信息学分析提示circMyt1l、rno-let-7d-5p、BDNF存在相互作用。OGD1、OGD2、OGD3 3组OPCs内circMyt1l的表达较Mock组均降低,差异均有统计学意义(P<0.01);而rno-let-7d-5p的表达较Mock组均升高,差异均有统计学意义(P<0.01);OGD1与Mock组间BDNF蛋白的表达差异无统计学意义(P>0.05),OGD2、OGD3两组与Mock组比较差异均有统计学意义(P<0.05)。结论: circMyt1l/rno-let-7d-5p/BDNF三者在OGD的OPCs中表达具有相互关系,circMyt1l表达的降低对rno-let-7d-5p的作用减弱,促进了其靶向BDNF的表达。

Objective: To investigate the expression and interaction of circMyt1l/rno-let-7d-5p/brain-derived neurotrophic factor(BDNF) in oligodendrocyte progenitor cells(OPCs). Methods: Bioinformatics analysis was used to analyze the differentially expressed circMyt1l and it's interacting miRNAs and mRNAs in the brain tissues of young rats with periventricular white matter injury. OPCs were isolated and cultured, and divided into control group(Mock group) and oxygen glucose deprivation(OGD) groups for 1 h, 2 h and 3 h, Namely OGD1, OGD2 and OGD3 groups. The expression levels of circMyt1l and rno-let-7d-5p genes were detected by qRT-PCR, and the expression levels of BDNF protein were detected by Western Blotting. Results: Bioinformatics analysis suggested that circMyt1l, rno-let-7d-5p and BDNF interact with each other. The expression of circMyt1l in OPCs in OGD1, OGD2 and OGD3 groups was lower than that in Mock group, and the differences were statistically significant(P<0.01).The expression of rno-let-7d-5p was higher than that of Mock group, and the differences were statistically significant(P<0.01). The expression of BDNF protein showed no significant difference between OGD1 and the Mock group(P>0.05), while there were significant differences between OGD2, OGD3 groups and the Mock group(P<0.05). Conclusion: The expression of circMyt1l/rno-let-7d-5p/BDNF is interrelated in OPCs of OGD. Decreased expression of circMyt1l weakened the effect on rno-let-7d-5p and promoted the expression of its targeted BDNF.

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