LRG1、miR-155表达水平在脓毒症治疗前后的变化及其对预后的评估价值-东南大学学报医学版

LRG1、miR-155表达水平在脓毒症治疗前后的变化及其对预后的评估价值

单位：1. 凉山彝族自治州第一人民医院急诊科, 四川西昌 615000;
2. 四川大学华西医院肾病内科, 四川成都 610047

分类号：R631

出版年·卷·期（页码）：2023·42·第四期（506-511）

摘要：

目的:探讨亮氨酸丰富α2-糖蛋白1(LRG1)、微小核糖核酸-155(miR-155)表达水平在脓毒症治疗前后的变化及其对预后评估价值。方法:回顾性分析2020年1月至2022年1月凉山彝族自治州第一人民医院收治的94例脓毒症患者的临床资料(设置为病例组),另选取本院同期106例健康体检者为对照组。对所有参与者均采集血液样本检测血清LRG1、miR-155、白细胞介素(IL)-6、C反应蛋白(CRP)、IL-8表达水平。比较脓毒症患者与健康体检者入院时血清LRG1、miR-155、IL-6、CRP、IL-8表达水平;比较不同病情、预后情况脓毒症患者的血清LRG1、miR-155、IL-6、CRP、IL-8表达水平;分析脓毒症患者血清LRG1、miR-155表达水平与血清IL-6、CRP、IL-8表达水平的相关性。结果:病例组血清LRG1、miR-155、IL-6、CRP、IL-8表达水平高于对照组(P＜0.05)。重度脓毒症患者入院时与入院第3天血清LRG1、miR-155、IL-6、CRP、IL-8表达水平高于轻、中度脓毒症患者(P＜0.05);预后较差脓毒症患者入院时血清LRG1、miR-155、IL-6、CRP、IL-8表达水平高于预后良好脓毒症患者(P＜0.05)。受试者工作特征(ROC)曲线分析结果显示,LRG1、miR-155预测脓毒症不良预后的曲线下面积分别为0.867和0.855。Pearson分析显示,脓毒症患者的血清LRG1、miR-155表达水平与血清IL-6、CRP、IL-8表达水平呈正相关(P＜0.05)。结论:LRG1、miR-155表达水平与脓毒症发生发展有关,LRG1、miR-155表达水平升高可导致脓毒症患者病情加重和预后不良。

Objective: To explore the abundance of leucine α Changes of the expression levels of 2-glycoprotein 1(LRG1) and microribonucleic acid-155(miR-155) in sepsis before and after treatment and their role in evaluating the prognosis. Methods: The clinical data of 94 patients with sepsis in the First People's Hospital of Liangshan Yi Autonomous Prefecture from January 2020 to January 2022 were retrospectively analyzed and set as the experimental group, and the data of 106 healthy people in the same institution during the same period were selected into control group. All participants were collected blood samples to detect the expression levels of serum LRG1, miR-155, interleukin-6(IL-6), C-reactive protein(CRP) and interleukin-8(IL-8). The levels of serum LRG1, miR-155, IL-6, CRP, IL-8 were compared between sepsis patients and healthy controls at admission. The levels of serum LRG1, miR-155, IL-6, CRP, IL-8 in sepsis patients with different conditions and prognosis were compared. The correlation between serum LRG1, miR-155 and the expression of IL-6, CRP, IL-8 in sepsis patients were analyzed. Results: Serum LRG1, miR-155, IL-6, CRP, IL-8 in the experimental group were higher than those in the control group(P＜0.05). Serum LRG1, miR-155, IL-6, CRP and IL-8 levels of patients with severe sepsis at admission and on the third day of admission were higher than those of patients with mild and moderate sepsis(P＜0.05); Serum LRG1, miR-155, IL-6, CRP, IL-8 in sepsis patients with poor prognosis were higher than those in sepsis patients with good prognosis(P＜0.05). The analysis of the receiver operating characteristic curve showed that the area under the curve of LRG1 and miR-155 for predicting poor prognosis of sepsis was 0.867 and 0.855, respectively. Pearson analysis showed that serum LRG1, miR-155 levels were positively correlated with serum IL-6, CRP, and IL-8 levels in sepsis patients(P＜0.05). Conclusion: The expression level of LRG1 and miR-155 is related to the occurrence and development of sepsis. The increased expression level of LRG1 and miR-155 can lead to the aggravation of sepsis and the decrease of prognosis quality.

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