宫颈癌组织KLK4、STAG2的表达及其临床意义-东南大学学报医学版

宫颈癌组织KLK4、STAG2的表达及其临床意义

单位：郑州大学附属肿瘤医院妇科, 河南郑州 450000

分类号：R737.33

出版年·卷·期（页码）：2023·42·第四期（540-546）

摘要：

目的:分析激肽释放酶4(KLK4)、基质抗原2(STAG2)在宫颈癌中的表达及其与临床病理特征及预后的关系。方法:选取2015年2月至2017年3月本院收治的397例宫颈癌患者为研究对象,在手术过程中收集癌组织及癌旁正常组织。检测组织KLK4 mRNA、STAG2 mRNA水平及KLK4、STAG2蛋白表达;采用Pearson相关系数检验KLK4 mRNA水平与STAG2 mRNA水平的相关性;采用Kaplan-Meier法绘制宫颈癌患者生存曲线;分析宫颈癌患者预后的影响因素。结果:与癌旁正常组织相比,宫颈癌组织KLK4 mRNA、STAG2 mRNA水平升高(P<0.05),且两者mRNA水平呈正相关(r=0.461,P<0.05)。宫颈癌组织KLK4蛋白阳性率、STAG2蛋白阳性率较癌旁正常组织高(P<0.05)。肿瘤直径＞5.0 cm、FIGO分期为Ⅲ+Ⅳ期、肌层浸润深度＞1/2的宫颈癌患者KLK4阳性表达比例、STAG2阳性表达比例均高于肿瘤直径≤5.0 cm、FIGO分期为Ⅰ＋Ⅱ期、肌层浸润深度≤1/2的患者(P＜0.05)。KLK4阴性组生存率高于KLK4阳性组(P=0.002),STAG2阴性组生存率高于STAG2阳性组(P＜0.001)。KLK4阳性表达、STAG2阳性表达、FIGO分期(Ⅲ~Ⅳ)、肌层浸润深度(＞1/2)是影响宫颈癌患者不良预后的独立危险因素(P＜0.05)。结论:宫颈癌组织KLK4、STAG2的mRNA水平及蛋白阳性表达率均显著高于癌旁组织,两者与肿瘤直径、FIGO分期、肌层浸润深度及预后密切相关。

Objective: To analyze the expression of kallikrein 4(KLK4) and matrix antigen 2(STAG2) in cervical cancer and their relationship with clinicopathological characteristics and prognosis. Methods: A total of 397 patients with cervical cancer who were collected in our hospital from February 2015 to March 2017 were regarded as the study objects. During the operation, cancer tissues and normal tissues adjacent to cancer were collected. KLK4 mRNA and STAG2 mRNA levels and expression of KLK4 and STAG2 proteins were detected; the correlation between KLK4 and STAG2 mRNA levels was determined; Kaplan-Meier method was used to draw the survival curve of cervical cancer patients; the prognostic factors of cervical cancer patients were analyzed. Results: The levels of KLK4 mRNA and STAG2 mRNA in cervical cancer tissues were higher than those in normal adjacent tissues(P<0.05), there was a positive correlation between the two mRNA levels(r=0.461,P<0.05). The positive rates of KLK4 protein and STAG2 protein in cervical cancer tissues were higher than those in normal adjacent tissues(P<0.05). The proportions of KLK4 positive expression and STAG2 positive expression in cervical cancer patients with tumor diameter >5.0 cm, FIGO stage Ⅲ+Ⅳ, and muscle invasion depth>1/2 were higher than those with tumor diameter ≤5.0 cm, FIGO stage Ⅰ+Ⅱ, and muscle invasion depth ≤1/2(P<0.05). The survival rate of KLK4 negative group was higher than that of KLK4 positive group(P=0.002), the survival rate of STAG2 negative group was higher than that of STAG2 positive group(P<0.001). KLK4 positive expression, STAG2 positive expression, FIGO stage(Ⅲ-Ⅳ), and depth of muscle invasion(>1/2) were independent risk factors for poor prognosis of cervical cancer patients(P<0.05). Conclusion: KLK4 and STAG2 mRNA levels and protein positive expression rates in cervical cancer tissues are obviously higher than those in adjacent tissues, and they are closely related to tumor diameter, FIGO stage, depth of muscle invasion and prognosis.

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