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非小细胞肺癌组织LncRNA CRNDE表达及临床意义
作者:张效亮  刘晓娇  马小婷  张超 
单位:中国人民解放军联勤保障部队第九四○医院 放射治疗科, 甘肃 兰州 730050
关键词:非小细胞肺癌 长链非编码RNA 结直肠肿瘤差异表达基因 TCGA 预后 
分类号:R734.2
出版年·卷·期(页码):2024·43·第二期(202-208)
摘要:

目的: 分析长链非编码RNA结直肠肿瘤差异表达基因(LncRNA CRNDE)在非小细胞肺癌(NSCLC)组织中的表达及临床病理意义。方法: 2020年2月至2023年5月,来自我院的63例NSCLC患者被纳入研究。采用实时定量聚合酶链反应(RT-qPCR)检测肿瘤组织和邻近正常组织中LncRNA CRNDE的表达。利用本研究队列和TCGA队列分析LncRNA CRNDE的临床病理意义和预后价值。结果: RT-qPCR分析显示,LncRNA CRNDE在肿瘤组织中的表达显著高于邻近的正常组织[3.04(0.56,13.04)vs. 0.24(0.05,1.99),P<0.001]。在受试者工作特征曲线中,LncRNA CRNDE是有能力区分NSCLC肿瘤组织和邻近正常组织的,曲线下面积为0.713(P<0.001),特异度较高,为81.0%。肿瘤组织LncRNA CRNDE在TNM Ⅲ期中表达水平高于Ⅰ~Ⅱ期患者(P=0.001)。TCGA数据显示,与邻近的正常组织(n=106)相比,LncRNA CRNDE在NSCLC肿瘤组织(n=1 014)中呈高表达[41.00(24.00,72.00)vs. 22.60(10.60,47.70),P<0.001]。LncRNA CRNDE高表达与所有患者较短的总生存(OS)时间以及肺鳞癌患者较短的OS时间和首次进展时间有关(P<0.05)。多变量生存分析结果也表明,N2/3分期(P=0.004)和LncRNA CRNDE高表达(P=0.010)与NSCLC患者较短的OS时间显著相关。结论: LncRNA CRNDE有希望作为NSCLC的潜在诊断标志物和治疗靶点。

Objective: To analyze the expression and clinical pathological significance of long non-coding RNA colorectal neoplasia differentially expressed(LncRNA CRNDE) in non-small cell lung cancer(NSCLC) tissues. Methods: From February 2020 to May 2023, 63 NSCLC patients from our hospital were included in the study. Real time quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression of LncRNA CRNDE in tumor tissues and adjacent normal tissues. Then, the clinical pathological significance and prognostic value of LncRNA CRNDE were analyzed using this study cohort and TCGA cohort. Results: Through RT-qPCR analysis, the expression of LncRNA CRNDE in tumor tissues was significantly higher than that in adjacent normal tissues [3.04(0.56, 13.04) vs. 0.24(0.05, 1.99), P<0.001]. In the receiver operating characteristic curve of the subjects, LncRNA CRNDE had the ability to distinguish between NSCLC tumor tissue and adjacent normal tissue, with an area under the curve of 0.713(P<0.001) and a high specificity of 81.0%. The expression level of LncRNA and CRNDE in tumor tissue was higher in TNM stage Ⅲ than that in patients with stage Ⅰ-Ⅱ(P=0.001). Similarly, by analyzing TCGA data, LncRNA CRNDE was highly expressed in NSCLC tumor tissue(n=1 014) compared to adjacent normal tissues(n=106) [41.00(24.00, 72.00) vs. 22.60(10.60, 47.70), P<0.001]. The high expression of LncRNA CRNDE was associated with a shorter overall survival(OS) time for all patients, as well as a shorter OS time and first progression time for lung squamous cell carcinoma patients(P<0.05). The results of multivariate survival analysis also showed that N2/3 staging(P=0.004) and high expression of LncRNA CRNDE(P=0.010) were significantly correlated with shorter OS time in NSCLC patients. Conclusion: LncRNA CRNDE has potential as a diagnostic marker and therapeutic target for NSCLC.

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